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Cluster of Differentiation 96 in Children with Acute Leukemia: A Single Center Cohort Study

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Cluster of differentiation 96 (CD96) is an important leukemic stem cells (LSCs) surface marker. We evaluated CD96 expression in children with acute leukemia (AL) and described its relation with treatment response. We conducted a prospective cohort study in Mansoura University Children's Hospital, Egypt during the period from 2014 to 2016. We studied 96 children with AL and 96 controls at clinical, laboratory and radiological levels. We assessed CD96% in LSCs using flow cytometry. AL group included 59 acute lymphoblastic leukemia (ALL) and 37 acute myeloid leukemia (AML) patients. ALL subgroup involved 44 B–ALL and 15 T–ALL patients while AML subgroup included 17 M2, 12 M4 and 8 M5 patients. CD96% was higher in AL group [57.63 (21.18–89.93)] than control [34.12 (16.15–39.51)] (P < 0.001). CD96% was higher in AML [68.25 (31.1–89.86)] than ALL [54.18 (21.18–89.93] (P < 0.001). CD96% in AML was M4 > M2 > M5 (P = 0.04) while within ALL subgroup, no significant difference was found between B–ALL and T–ALL (P = 0.807). CD96% in patients with non-complete remission was higher than those with complete remission (P = 0.004). CD96 is a reliable diagnostic marker for AL mainly AML and could be used as a prognostic marker for treatment response.

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Fig. 1

Abbreviations

AL:

Acute leukemia

ALL:

Acute lymphoblastic leukemia

AML:

Acute myeloid leukemia

BM:

Bone marrow

CD:

Cluster of differentiation

CR:

Complete remission

DAPK:

Death Associated Protein Kinase

EDTA:

Ethylenediaminetetraacetic acid

FAB:

French–American–British

FACS:

Fluorescence-activated cell sorting

LSCs:

Leukemic stem cells

MAL:

Mixed lineage acute leukemia

NCR:

Non-complete remission

NK:

Natural killer

PBS:

Phosphate-buffered saline

PCR:

Polymerase chain reaction

RBCs:

Red blood cells

SE:

Standard error

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Acknowledgements

We greatly acknowledge the patients and their families for their patience and help.

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Authors and Affiliations

  1. Genetic Unit, Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt

    Hanan Mohammad, Yahya Wahba & Ali Shaltout

  2. Section of Molecular Immunology, Mansoura University Children’s Hospital, Mansoura, Egypt

    Mona Gouida

Authors
  1. Hanan Mohammad
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  2. Yahya Wahba
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  3. Mona Gouida
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  4. Ali Shaltout
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Contributions

Hanan Mohammad: Collection of clinical data, revising the article draft and final approval of the version to be submitted. Yahya Wahba: Idea of the research, conception and design of the study, interpretation of clinical and laboratory data, drafting the article and final approval of the version to be submitted. Mona Gouida: Analysis of the laboratory data, revising the article draft and final approval of the version to be submitted. Ali Shaltout: Analysis of clinical and laboratory data, conception of the study, drafting the article and final approval of the version to be submitted.

Corresponding author

Correspondence to Yahya Wahba.

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Authors declared that they have no conflict of interest.

Ethical Standards

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee of Faculty of Medicine, Mansoura University, Egypt (Code No: MS/804) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consents were obtained from all individual participants included in the study.

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Mohammad, H., Wahba, Y., Gouida, M. et al. Cluster of Differentiation 96 in Children with Acute Leukemia: A Single Center Cohort Study. Indian J Hematol Blood Transfus 36, 178–182 (2020). https://doi.org/10.1007/s12288-019-01145-2

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  • DOI: https://doi.org/10.1007/s12288-019-01145-2

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