This work aims to clarify how blood coagulation parameters reflect mild stress response in males and females. Healthy student volunteers of both sexes were used in this pilot study. A new global sensitive assay of haemostasis, spatial thrombodynamics, along with conventional coagulometry approach were used to evaluate of blood coagulation parameters. Psychodiagnostics scales (according to Spielberger and Taylor) are employed to evaluate anxiety as stress-induced response. We have selected exam stress, which despite being a mild stressor may nevertheless cause somatic disorders. We provide the first evidence of a statistically significant increase in initial clot growth velocity in women, but not men, in response to exam stress. The exam situation produces higher situational anxiety in female volunteers, and so they express remarkable stress-induced haemostatic responses, including plasma- and platelet-based changes. In contrast, male volunteers do not express pronounced stress-induced changes in haemostasis, and only display a decrease in plateletcrit value and an increase in prothrombin time. Mild form of stress (exam) induces changes in some blood coagulation parameters. A statistically significant remarkable increase in Vinit value and some other plasma- and platelet-based parameters has been seen in female students (but not male ones) under exam stress.
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- Vinit :
The initial velocity of induced thrombosis
- Vsteady :
The steady velocity of induced thrombosis
- dSpeed :
The difference between the initial and steady velocity of induced thrombosis
International normalized ratio
Activated partial thromboplastin time
Absolute number of platelets
The average platelets’ volume
State-trait anxiety inventory
Teilor’s Manifest Anxiety Scale
von Känel R, Vökt F, Biasiutti FD, Stauber S, Wuillemin WA, Lukas PS (2012) Relation of psychological distress to the international normalized ratio in patients with venous thromboembolism with and without oral anticoagulant therapy. J Thromb Haemost 10(8):1547–1555. https://doi.org/10.1111/j.1538-7836.2012.04801.x
Stämpfli SF, Camici GG, Keller S, Rozenberg I, Arras M, Schuler B, Gassmann M, Garcia I, Lüscher TF, Tanner FC (2014) Restraint stress enhances arterial thrombosis in vivo–role of the sympathetic nervous system. Stress 17(1):126–132. https://doi.org/10.3109/10253890.2013.862616
von Känel R (2015) Acute mental stress and hemostasis: when physiology becomes vascular harm. Thromb Res 135(Suppl 1):S52–S55. https://doi.org/10.1016/S0049-3848(15)50444-1
Tofler GH, Stone PH, Maclure M, Edelman E, Davis VG, Robertson T et al (1990) Analysis of possible triggers of acute myocardial infarction (the MILIS study). Am J Cardiol 66:22–27. https://doi.org/10.1016/0002-9149(90)90729-K
Mostofsky E, Penner EA, Mittleman MA (2014) Outbursts of anger as a trigger of acute cardiovascular events: a systematic review and meta-analysis. Eur Heart J 35:1404–1410. https://doi.org/10.1093/eurheartj/ehu033
Steptoe A, Strike PC, Perkins-Porras L, McEwan JR, Whitehead DL (2006) Acute depressed mood as a trigger of acute coronary syndromes. Biol Psychiatry 60:837–842. https://doi.org/10.1016/j.biopsych.2006.03.041
Zunhammer M, Eberle H, Eichhammer P, Busch V (2013) Somatic symptoms evoked by exam stress in university students: the role of alexithymia, neuroticism, anxiety and depression. PLoS ONE 8(12):e84911. https://doi.org/10.1371/journal.pone.0084911
Weekes N, Lewis R, Patel F, Garrison-Jakel J, Berger DE, Lupien SJ (2006) Examination stress as an ecological inducer of cortisol and psychological responses to stress in undergraduate students. Stress 9(4):199–206. https://doi.org/10.1080/10253890601029751
Miller VM, Kaplan JR, Schork NJ, Ouyang P, Berga SL, Wenger NK, Shaw LJ, Webb RC, Mallampalli M, Steiner M, Taylor DA, Merz CN, Reckelhoff JF (2011) Strategies and methods to study sex differences in cardiovascular structure and function: a guide for basic scientists. Biol Sex Differ 2:14. https://doi.org/10.1186/2042-6410-2-14
Davydov DM, Zhdanov RI, Dvoenosov VG, Kravtsova OA, Voronina EN, Filipenko ML (2015) Resilience to orthostasis and haemorrhage: a pilot study of common genetic and conditioning mechanisms. Sci Rep 5:10703. https://doi.org/10.1038/srep10703
Tuktamyshov RN, Zhdanov RI (2015) The method of in vivo evaluation of haemostasis: spatial thrombodynamics. Hematology 20(10):584–586. https://doi.org/10.1179/1607845415Y.0000000022
Lipets E, Ataullakhanov F (2015) Global assays of hemostasis in the diagnostics of hypercoagulation and evaluation of thrombosis risk. Thromb J 13(4):2–15. https://doi.org/10.1186/s12959_015_0038_0
Sinauridze EI, Gorbatenko AS, Seregina EA, Lipets EN, Ataullakhanov FI (2017) Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation. Sci Rep 7(1):843. https://doi.org/10.1038/s41598-017-00927-w
Kupriyanov RV, Zhdanov RI (2014) Stress and allostasis: problems, outlooks and relationships. IP Pavlov J Higher Nerv Act (Zhurnal Vysshei Nervnoi Deyatelnosti imeni IP Pavlova) 64(1):21–31. https://doi.org/10.7868/S0044467714010080
Veldhuijzen van Zanten JJ, Ring C, Burns VE, Edwards KM, Drayson M, Carroll D (2004) Mental stress- induced hemoconcentration: sex differences and mechanisms. Psychophysiology 41(4):541–551. https://doi.org/10.1111/j.1469-8986.2004.00190.x
The authors are grateful to Professor Dr. Fazly Ataullakhanov (Lomonosov Moscow State University and “HemaCore” Ltd) for the support with thrombodynamics studies and fruitful discussions. The work was supported and 2016-2018 Grant of Russian Institute of Advanced Study at the Moscow State Pedagogical University (to R.V.K.).
This work was funded by the subsidy allocated by the Russian Ministry of Education and Science, Grant # 19.9777.2017/8.9 to Kazan Federal University for the state assignment in the sphere of scientific activities.
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards approved by the Local Ethics Committee of the Kazan State Medical University (6.21.06.18) and with the 1964 Helsinki declaration and its later amendments.
Informed consent was obtained from all individual participants included in the study.
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Zhdanov, R.I., Kupriyanov, R.V., Zhdanova, S.I. et al. Relationship Between Haemostasis Parameters and Anxiety Under Examination Stress: A Pilot Study. Indian J Hematol Blood Transfus 34, 727–730 (2018). https://doi.org/10.1007/s12288-018-0980-7
- Blood coagulation