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Cupincin: A Novel Cupin Domain Containing Protease from Rice (Oryza sativa L.) Bran Comprising of Procoagulant and Fibrinogenolytic Activity

  • Roopesh Sreedhar
  • Purnima Kaul Tiku
Original Article

Abstract

The current study was carried out to evaluate the pharmacological properties of cupincin- A novel cupin domain containing metalloprotease with limited proteolysis from rice bran on blood coagulation and hydrolysis of human fibrinogen. Cupincin preferentially hydrolyzed the Aα chain of fibrinogen and then the Bβ-chain, but not the γ-chain. Cupincin reduced the re-calcification time of citrated human plasma dose dependently. Analysis of citrated whole blood in the presence of cupincin by rotem showed a decrease in coagulation time and clot formation time. Sonoclot analysis indicated that cupincin cleaved fibrinogen of whole citrated blood. SDS-PAGE and sonoclot analysis (LI-30) indicated that cupincin lacked plasmin-like activity. Global hemostasis tests like rotem and sonoclot analysis determined cupincin as a procoagulant enzyme. Cupincin did not show any effect on prothrombin time and activated partial thromboplastin time tests suggesting its action on the common pathway of coagulation. The involvement of proteases from rice (Oryza sativa L.) in haemostasis has never been exploited before. This study could provide the basis for the development of new procoagulant agents from a nontoxic source like rice.

Keywords

Rice bran Protease Procoagulant Fibrinogenolytic Haemostasis Thromboelastometry 

Notes

Acknowledgements

We are grateful to Director, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, for his support and encouragement. The authors wish to thank Vilas Hiremath of Vijayashree diagnostic centre, Bengaluru for his help in Thromboelastometry and Sonoclot analysis. Roopesh S acknowledges the award of Senior Research Fellowship from Indian Council of Medical Research, New Delhi.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Indian Society of Haematology & Transfusion Medicine 2017

Authors and Affiliations

  1. 1.Department of Protein Chemistry and TechnologyCSIR-Central Food Technological Research InstituteMysuruIndia

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