Abstract
Human parvovirus B19 is highly tropic to human bone marrow and replicates only in erythroid progenitor cells. It is causative agent of transient aplastic crisis in patients with chronic haemolytic anemia. In immunocompromised patients persistent parvovirus B19 infection may develop and it manifests as pure red cell aplasia and chronic anaemia. Bone marrow is characterised morphologically by giant pronormoblast stage with little or no further maturation. We encountered a case of 6 year old HIV positive male child presented with pure red cell aplasia due to parvovirus B19 infection. Bone marrow aspiration cytology revealed giant pronormoblast with prominent intranuclear inclusions led to suspicion of parvovirus B19 infection which was confirmed by DNA PCR. This case is presented to report classical morphological features of parvovirus B19 infection rarely seen on bone marrow examination should warrant the suspicion of human parvovirus B19 infection in the setting of HIV positive patient with repeated transfusions and confirmation should be done by PCR.
References
Brown KE, Young NS (1995) Parvovirus B19 infection and hematopoiesis. Blood Rev 9:176–182
Cossart YE, Field AM, Cant B, Widdow D (1975) Parvovirus like particles in human sera. Lancet 1(7898):72–73
Brown KE, Anderson SM, Young NS (1993) Erythrocyte P antigen: cellular receptor for B19 parvovirus. Science 262:114–117
Wan Z, Zhi N, Wong S, Keyvanfar K, Liu D, Raghavachari N, Munson P, Su S, Malide D, Kajigaya S, Young N (2010) Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors. J Clin Invest 120(10):3530–3544
Galel SA, Malone JM, Vivele MK.Transfusion Medicine. In: Greer JP, Rodgers GM, Foerster J, Paraskevas F, Lukens JN, Glader B.Wintrobe′s clinical hematology. 11th ed. Philadelphia:Lippincott Williams & Wilkins;2004. p.869-870
Brown KE, Young NS (1996) Parvovirus and bone marrow failure. Stem cells 14:151–163
Abkowitz JL, Brown KE, Wood RW, Kovach NL, Green SW, Young NS (1997) Clinical relevance of parvovirus B19 as a cause of anemia in patients with Human Immunodeficiency virus infection. J Infect Dis 176:269–273
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sharada Raju, R., Nalini Vinayak, K., Madhusudan Bapat, V. et al. Acute Human Parvovirus B19 Infection: Cytologic Diagnosis. Indian J Hematol Blood Transfus 30 (Suppl 1), 133–134 (2014). https://doi.org/10.1007/s12288-013-0287-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12288-013-0287-7