Skip to main content

Advertisement

SpringerLink
Log in

Preparation, characterization, and in vitro cytotoxicity activity of allyl-isothiocyanate-embedded polymeric nanoparticles for potential breast cancer targeting

  • Original Article
  • Published:
Breast Cancer Aims and scope Submit manuscript

Abstract

Background

Allyl isothiocyanate (AITC) is an excellent active phytoconstituent recently revealed for cancer treatment. The strategic prominence of this study was to synthesize and characterize AITC-embedded tripolyphosphate-modified chitosan nanoparticles (AITC@CS-TPP-NPs) by ionic gelation.

Method

Chitosan is recycled as a polymer to fabricate AITC@CS-TPP-NPs; the fabricated nanoparticles (NPs) are then characterized using FT-IR spectroscopy, DSC, XRD, zeta potential, size analysis, SEM, EDX, entrapment efficiency, in vitro drug release study, and in vitro cytotoxicity activity against MCF-7 to explore the effectiveness and strength.

Results

As a result, developed AITC@CS-TPP-NPs demonstrates good stability with a zeta potential of 35.83 mV and 90.14% of drug release. The anticancer potential of AITC@CS-TPP-NPs shows the improved cytotoxicity activity of AITC due to the surface modification of CS using TPP. Hence, the cytotoxicity of AITC@CS-TPP-NPs was tested in vitro against a human breast cancer cell line (MCF-7) and found to be considerable.

Conclusion

The AITC@CS-TPP-NPs were effectively synthesized and have significant benefits, including being easy to prepare, stable, and affordable with wide use in human breast cancer against cell line (MCF-7).

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

Data availability

Not applicable.

References

  1. Ballauff M, Lu Y. “Smart” nanoparticles: preparation, characterization and applications. Polymer. 2007;48(7):1815–23.

    Article  CAS  Google Scholar 

  2. Sawtarie N, Cai Y, Lapitsky Y. Preparation of chitosan/tripolyphosphate nanoparticles with highly tunable size and low polydispersity. Colloids Surf, B. 2017;157:110–7.

    Article  CAS  Google Scholar 

  3. Najminejad Z, et al. Clinical perspective: antibody-drug conjugates (ADCs) for the treatment of HER2-positive breast cancer. Mol Ther. 2023. https://doi.org/10.1016/j.ymthe.2023.03.019.

    Article  PubMed  Google Scholar 

  4. Debela DT, et al. New approaches and procedures for cancer treatment: current perspectives. SAGE Open med. 2021;9:20503121211034370.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Moo T-A, et al. Overview of breast cancer therapy. PET Clin. 2018;13(3):339–54.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Schirrmacher V. From chemotherapy to biological therapy: a review of novel concepts to reduce the side effects of systemic cancer treatment. Int J Oncol. 2019;54(2):407–19.

    Article  CAS  PubMed  Google Scholar 

  7. Mandracchia D, Tripodo G. Micro and nano-drug delivery systems. London: The Royal Society of Chemistry; 2021. p. 1–24.

    Google Scholar 

  8. Mohammed MA, et al. An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics. 2017;9(4):53.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Lepeltier E, Bourgaux C, Couvreur P. Nanoprecipitation and the “Ouzo effect”: application to drug delivery devices. Adv Drug Deliv Rev. 2014;71:86–97.

    Article  CAS  PubMed  Google Scholar 

  10. Ahmed TA, Aljaeid BM. Preparation, characterization, and potential application of chitosan, chitosan derivatives, and chitosan metal nanoparticles in pharmaceutical drug delivery. Drug Des Dev Ther. 2016;10:483.

    Article  CAS  Google Scholar 

  11. Matshetshe KI, et al. Preparation, characterization and in vitro release study of β-cyclodextrin/chitosan nanoparticles loaded Cinnamomum zeylanicum essential oil. Int J Biol Macromol. 2018;118:676–82.

    Article  CAS  PubMed  Google Scholar 

  12. Jonassen H, Kjøniksen A-L, Hiorth M. Stability of chitosan nanoparticles cross-linked with tripolyphosphate. Biomacromol. 2012;13(11):3747–56.

    Article  CAS  Google Scholar 

  13. Patil PB, Patel JK. Chemopreventive aspects, investigational anticancer applications and current perspectives on allyl isothiocyanate (AITC): a review. Mol Cell Biochem. 2023. https://doi.org/10.1007/s11010-023-04697-0.

    Article  PubMed  Google Scholar 

  14. Rajakumar T, et al. Effect of allyl isothiocyanate on NF-κB signaling in 7, 12-dimethylbenz (a) anthracene and N-methyl-N-nitrosourea-induced mammary carcinogenesis. Breast Cancer. 2018;25:50–9.

    Article  PubMed  Google Scholar 

  15. Chakraborty S, Bhattacharjee P. Supercritical carbon dioxide extraction of melatonin from Brassica campestris: in vitro antioxidant, hypocholesterolemic and hypoglycaemic activities of the extracts. Int J Pharm Sci Res. 2017;8(6):2486–95.

    CAS  Google Scholar 

  16. Ghadi A, et al. Synthesis and optimization of chitosan nanoparticles: Potential applications in nanomedicine and biomedical engineering. Caspian J Intern Med. 2014;5(3):156.

    PubMed  PubMed Central  Google Scholar 

  17. Esquivel R, et al. Synthesis and characterization of new thiolated chitosan nanoparticles obtained by ionic gelation method. Int J Polym Sci. 2015. https://doi.org/10.1155/2015/502058.

    Article  Google Scholar 

  18. Joseph JJ, Sangeetha D, Gomathi T. Sunitinib loaded chitosan nanoparticles formulation and its evaluation. Int J Biol Macromol. 2016;82:952–8.

    Article  CAS  PubMed  Google Scholar 

  19. Ahmad MZ, et al. Progress in nanomedicine-based drug delivery in designing of chitosan nanoparticles for cancer therapy. Int J Polym Mater Polym Biomater. 2022;71(8):602–23.

    Article  CAS  Google Scholar 

  20. Encinas-Basurto D, et al. Poly (lactic-co-glycolic acid) nanoparticles for sustained release of allyl isothiocyanate: characterization, in vitro release and biological activity. J Microencapsul. 2017;34(3):231–42.

    Article  CAS  PubMed  Google Scholar 

  21. Singh A, Mittal A, Benjakul S. Chitosan nanoparticles: preparation, food applications and health benefits. Sci Asia. 2021;47:1–10.

    Article  CAS  Google Scholar 

  22. Perera U, Rajapakse N. Chitosan nanoparticles: preparation, characterization, and applications. In: Seafood processing by-products. Springer; 2014. p. 371–87.

    Chapter  Google Scholar 

  23. Bugnicourt L, Ladavière C. Interests of chitosan nanoparticles ionically cross-linked with tripolyphosphate for biomedical applications. Prog Polym Sci. 2016;60:1–17.

    Article  CAS  Google Scholar 

  24. Kong J, Yu S. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin. 2007;39(8):549–59.

    Article  CAS  PubMed  Google Scholar 

  25. Khan ZG, Vaidehi BS, Sarang GN, Shivam GB, Patil PB. Second Order Derivative Spectrophotometric Method for Estimation of Lurasidone in Bulk and Pharmaceutical Formulation. J. Pharm Sci. Tech. 2017;7(1):02–5.

  26. Pandey AP, et al. Nanoarchitectonics in cancer therapy and imaging diagnosis. J Nanosci Nanotechnol. 2014;14(1):828–40.

    Article  CAS  PubMed  Google Scholar 

  27. Pham DT, Saelim N, Tiyaboonchai W. Crosslinked fibroin nanoparticles using EDC or PEI for drug delivery: physicochemical properties, crystallinity and structure. J Mater Sci. 2018;53(20):14087–103.

    Article  CAS  Google Scholar 

  28. Caputo F, et al. Measuring particle size distribution of nanoparticle enabled medicinal products, the joint view of EUNCL and NCI-NCL. A step by step approach combining orthogonal measurements with increasing complexity. J Controll Release. 2019;299:31–43.

    Article  CAS  Google Scholar 

  29. Hosseini SF, et al. Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: preparation, characterization and in vitro release study. Carbohyd Polym. 2013;95(1):50–6.

    Article  CAS  Google Scholar 

  30. Nair RS, et al. An evaluation of curcumin-encapsulated chitosan nanoparticles for transdermal delivery. AAPS PharmSciTech. 2019;20(2):1–13.

    Article  Google Scholar 

  31. D’Souza S. A review of in vitro drug release test methods for nano-sized dosage forms. Adv Pharm. 2014. https://doi.org/10.1155/2014/304757

    Article  Google Scholar 

  32. Houghton P, et al. The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity. Methods. 2007;42(4):377–87.

    Article  CAS  PubMed  Google Scholar 

  33. Wen L-L, et al. Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene. Enzyme Microb Technol. 2016;85:19–24.

    Article  CAS  PubMed  Google Scholar 

  34. Garg U, et al. Current advances in chitosan nanoparticles based drug delivery and targeting. Adv Pharma Bull. 2019;9(2):195.

    Article  CAS  Google Scholar 

  35. Cai L, et al. β-Cyclodextrin-allyl isothiocyanate complex as a natural preservative for strand-based wood composites. Compos B Eng. 2020;193: 108037.

    Article  CAS  Google Scholar 

  36. Encinas-Basurto D, et al. Targeted drug delivery via human epidermal growth factor receptor for sustained release of allyl isothiocyanate. Curr Top Med Chem. 2018;18(14):1252–60.

    Article  CAS  PubMed  Google Scholar 

  37. Kim W-T, et al. Characterization of calcium alginate and chitosan-treated calcium alginate gel beads entrapping allyl isothiocyanate. Carbohyd Polym. 2008;71(4):566–73.

    Article  CAS  Google Scholar 

  38. Bansal P, et al. In vitro anticancer activity of dietary bioagent (isothiocyanates) on HepG2 and B16F10 cell lines: a comparative study. Ann Plant Sci. 2013;2(7):234–7.

    Google Scholar 

  39. Pawlik A, et al. Sulforaphene, an isothiocyanate present in radish plants, inhibits proliferation of human breast cancer cells. Phytomedicine. 2017;29:1–10.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors express acknowledgment of their gratitude to the Management and Dean of the Faculty of Pharmacy, Nootan Pharmacy College, Sankalchand Patel University, Visnagar (Gujarat), as well as the Management and Principal of the H. R. Patel Institute of Pharmaceutical Education & Research, Shirpur (Maharashtra) for providing the necessary resources to complete the study.

Author information

Authors and Affiliations

  1. Faculty of Pharmacy, Nootan Pharmacy College, Sankalchand Patel University, Visnagar, Gujarat, 384315, India

    Prashant Bhagwan Patil & Jayvadan Kantilal Patel

  2. Department of Pharmaceutical Chemistry, H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur, Maharashtra, 425405, India

    Prashant Bhagwan Patil

  3. Aavis Pharmaceuticals, Hoschton, GA, 30548, USA

    Jayvadan Kantilal Patel

Authors
  1. Prashant Bhagwan Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jayvadan Kantilal Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Prashant Bhagwan Patil.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Patil, P.B., Patel, J.K. Preparation, characterization, and in vitro cytotoxicity activity of allyl-isothiocyanate-embedded polymeric nanoparticles for potential breast cancer targeting. Breast Cancer 30, 1065–1078 (2023). https://doi.org/10.1007/s12282-023-01501-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12282-023-01501-1

Keywords

Search

Navigation