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Variant in BCAR4 gene correlated with the breast cancer susceptibility and mRNA expression of lncRNA BCAR4 in Chinese Han population

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Abstract

Background

Long non-coding RNA (LncRNA) Breast cancer anti-estrogen resistance 4 (BCAR4) has been shown to participate in the biological progress of various cancers including breast cancer. Genetic Polymorphisms in BCAR4 may have an influence on the progress of breast cancer, but it is rarely studied.

Methods

In our epidemiology study, three tagging SNPs (rs4561483, rs11649623 and rs13334967) in lncRNA BCAR4 were selected for genotyping among 487 breast cancer cases and 489 healthy controls. And quantitative real-time PCR (qRT-PCR) was performed to evaluate the relative mRNA expression of BCAR4 in different genotypes of the significant locus rs13334967.

Results

We found that BCAR4 rs13334967 is associated with lower breast cancer risk both in codominant model (AT vs AA, OR 0.632, 95% CI 0.429–0.931, TT vs AA, OR 0.731, 95% CI 0.511–0.990) and dominant model (AT + TT vs AA, OR 0.798, 95% CI 0.571–0.970). The further results of qRT-PCR displayed that carriers with rs13334967 AT, TT genotype have lower BCAR4 mRNA expression compared with AA genotype.

Conclusion

The research study implied that BCAR4 rs13334967 was correlated with the susceptibility to breast cancer and may impact the mRNA expression of BCAR4. LncRNA BCAR4 may be a potential biomarker and therapeutic target for breast cancer.

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Acknowledgements

The authors would like to thank the participants, the coordinators, and administrators for their supports during the study.

Funding

This work was supported by Henan university science and technology innovation talents support program (19HASTIT005); Medical Science and Technology key projects of Henan Province and Zhengzhou (192102310088, 19A32000820, SBGJ2018089). The National Natural Science Foundation of China (U1604168).

Author information

Author notes

  1. Rui Peng and Jingjing Cao contributed equally to this work and should be considered as co-first authors.

Authors and Affiliations

  1. Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, People’s Republic of China

    Rui Peng, Qiuyu Sun & Chunhua Song

  2. Henan Key Laboratory of Tumor Epidemiology, Zhengzhou, 450052, Henan, People’s Republic of China

    Qiuyu Sun & Chunhua Song

  3. Department of Teaching and Research, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, People’s Republic of China

    Rui Peng

  4. Department of Preventive Medicine, Heze Medical College, Heze, 274000, Shandong, People’s Republic of China

    Jingjing Cao

  5. Department for Endemic Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Zhengzhou, 450016, Henan, People’s Republic of China

    Qiaoyun Guo

  6. Department of Teaching and Research, Henan Tumor Hospital, Zhengzhou, 450008, Henan, People’s Republic of China

    Linping Xu

  7. Department of Anesthesiology, The First Affifiliated Hospital of Henan University of Science and Technology (New District Hospital), Guanlin Road and Xuefu Street Intersection in Luolong District, Luoyang, 471300, Henan, People’s Republic of China

    Xiaojuan Xie

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  1. Rui Peng
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Contributions

Conceptualization: RP, JC, QG, QS, LX, XX, CS. Validation: RP, JC, XX, CS. Formal analysis: RP, JC. Investigation: RP, JC. Writing-original draft: RP, JC. Writing-review and editing: RP, JC, QG, QS, LX, XX, CS. Visualization: QG, QS, LX. Supervision: XX, CS. Project administration: XX, CS.

Corresponding authors

Correspondence to Xiaojuan Xie or Chunhua Song.

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The authors declare no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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The manuscript has not been submitted to more than one journal for simultaneous consideration. The manuscript has not been published previously (partly or in full). A single study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support your conclusions. No data, text, or theories by others are presented as if they were the author’s own.

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Peng, R., Cao, J., Guo, Q. et al. Variant in BCAR4 gene correlated with the breast cancer susceptibility and mRNA expression of lncRNA BCAR4 in Chinese Han population. Breast Cancer 28, 424–433 (2021). https://doi.org/10.1007/s12282-020-01174-0

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  • DOI: https://doi.org/10.1007/s12282-020-01174-0

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