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A Comprehensive Analysis of MALDI-TOF MS and Ribosomal DNA Sequencing for Identification of Clinical Yeasts

  • Clinical Mycology Lab Issues (S Córdoba, Section Editor)
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Abstract

Purpose of Review

The purpose of this study is to analyze the current knowledge about matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) identification of clinical yeasts comparing with the ribosomal DNA sequencing identification. We also review the lasts taxonomical and nomenclature changes of clinical yeasts.

Recent Findings

In most studies, the two main commercially available, MALDI Biotyper and VITEK MS, correctly identified more than 90% of yeasts isolates. Most drawbacks are due to low discrimination between closely related species or due to a lack of the reference main spectra (MSP) in the database. The addition of MSPs in the databases improves the performance.

Summary

MALDI-TOF MS has proven to be good for the identification of clinical yeasts and to differentiate some closely related species or species undifferentiated by conventional techniques. However, some species remain difficult to differentiate by this technique and should be identified by ribosomal DNA sequencing. Accurate and rapid yeasts identification could improve the management of patients with yeasts infections.

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Acknowledgments

We thank Nadia Bueno for the writing assistance and Ruben Barrios for the technical support.

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Authors and Affiliations

  1. Departamento Micología, Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”, AV. Velez Sarsfield 563, Buenos Aires, Argentina

    Constanza Giselle Taverna, Mariana Mazza, Nicolas Refojo & Graciela Davel

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  1. Constanza Giselle Taverna
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  2. Mariana Mazza
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Correspondence to Constanza Giselle Taverna.

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Taverna, C.G., Mazza, M., Refojo, N. et al. A Comprehensive Analysis of MALDI-TOF MS and Ribosomal DNA Sequencing for Identification of Clinical Yeasts. Curr Fungal Infect Rep 11, 184–189 (2017). https://doi.org/10.1007/s12281-017-0297-2

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