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•• Arendrup MC, Cuenca-Estrella M, Lass-Flörl C, Hope WW, The European Committee on Antimicrobial Susceptibilty Testing Subcommittee on Antifungal Testing (EUCAST-AFST). EUCAST technical note on Aspergillus and amphotericin B, itraconazole, and posaconazole. Clin Microbiol Infect. In Press; doi:10.1111/j.1469-0691.2012.03890.x. First official breakpoints for Aspergillus species.
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/L98H resistant genotype among resistant isolates, b) demonstration of a variety of other non-synonomous mutations, and c) utility of a Cyp51A protein homology model to correlate individual mutations to their potential role for azole susceptibility.
• Snelders E, Karawajczyk A, Verhoeven RJ, et al. The structure-function relationship of the Aspergillus fumigatus cyp51A L98H conversion by site-directed mutagenesis: the mechanism of L98H azole resistance. Fungal Genet Biol. 2011;48:1062–70. Molecular dynamics simulations combined with site-directed mutagenesis of amino acid substitutions in the CYP51A gene demonstrate that both the L98H substitution and the 34 bp TR are needed to obtain the multi-azole resistant phenotype.
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/L98H in CF patients. Genotyping suggested selection for resistance in the patient as well as resistance being achieved in the environment as 1) susceptible and resistant isolates (not involving TR
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