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Syntaxin17 Restores Lysosomal Function and Inhibits Pyroptosis Caused by Acinetobacter baumannii

  • Microbial Pathogenesis and Host-Microbe Interaction
  • Published:
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Abstract

Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function. This study aimed to explore the role of STX17 in the regulation of pyroptosis induced by A. baumannii. Our findings indicate that overexpression of STX17 enhances autophagosome degradation, increases LAMP1 expression, reduces Cathepsin B release, and improves lysosomal function. Conversely, knockdown of STX17 suppresses autophagosome degradation, reduces LAMP1 expression, augments Cathepsin B release, and accelerates lysosomal dysfunction. In instances of A. baumannii infection, overexpression of STX17 was found to improve lysosomal function and reduce the expression of mature of GSDMD and IL-1β, along with the release of LDH, thus inhibiting pyroptosis caused by A. baumannii. Conversely, knockdown of STX17 led to increased lysosomal dysfunction and further enhanced the expression of mature of GSDMD and IL-1β, and increased the release of LDH, exacerbating pyroptosis induced by A. baumannii. These findings suggest that STX17 regulates pyroptosis induced by A. baumannii by modulating lysosomal function.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 81900074)

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Authors and Affiliations

  1. Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People’s Republic of China

    Zhiyuan An

  2. Department of Clinical Laboratory, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, 100730, People’s Republic of China

    Wenyi Ding

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  1. Zhiyuan An
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Correspondence to Zhiyuan An.

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An, Z., Ding, W. Syntaxin17 Restores Lysosomal Function and Inhibits Pyroptosis Caused by Acinetobacter baumannii. J Microbiol. (2024). https://doi.org/10.1007/s12275-024-00109-0

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