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Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. Isolated from the Aquatic Environment, South Korea

Journal of Microbiology volume 61, pages 37–47 (2023)Cite this article

Abstract

Two novel bacterial species CJ51T and CJ63T belonging to the genus Chryseobacterium were isolated from the Upo wetland and the Han River, South Korea, respectively. Cells of these strains were Gram-stain-negative, aerobic, non-motile, rod-shaped, and catalase- and oxidase-positive. Both strains were shown to grow optimally at 30 °C and pH 7 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ51T and CJ63T belonged to the genus Chryseobacterium and were most closely related to Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316sT with 98.47% and 98.46% 16S rRNA sequence similarities, respectively. The average nucleotide identity values of strains CJ51T and CJ63T with its closely related type strains Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316sT were 81.9% and 82.1%, respectively. The major fatty acids of strains CJ51T and CJ63T were iso-C15:0, iso-C17:0 3-OH and summed feature 9 (C16:0 10-methyl and/or iso-C17:1ω9c). Menaquinone 6 (MK-6) was identified as the primary respiratory quinone in both strains. The major polar lipids of strains CJ51T and CJ63T were phosphatidylethanolamine and several unidentified amino lipids and lipids. Based on polyphasic taxonomy data, strains CJ51T and CJ63T represent novel species of the genus Chryseobacterium, for which names Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. are proposed respectively. The type strains are CJ51T (= KACC 22749T = JCM 35632T) and CJ63T (= KACC 22750T = JCM 35633T).

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Acknowledgements

This work was supported by the National Institute of Biological Resources funded by the Ministry of Environment (No. NIBR202203205) and by the Korea Ministry of Environment (MOE) as ‘the Environmental Health Action Program (2016001350004)’. This research was supported by the Chung-Ang University Graduate Research Scholarship in 2022. We thank Professor Aharon Oren for his assistance with nomenclature. We thank J. Kim for technical help at the BT research center, Chung-Ang University.

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  1. Miryung Kim and Yong-Seok Kim contributed equally to this work.

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  1. Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea

    Miryung Kim, Yong-Seok Kim & Chang-Jun Cha

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Kim, M., Kim, YS. & Cha, CJ. Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. Isolated from the Aquatic Environment, South Korea. J Microbiol. 61, 37–47 (2023). https://doi.org/10.1007/s12275-022-00008-2

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