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Effects of multi-species probiotic supplementation on alcohol metabolism in rats

  • Microbial Physiology and Biochemistry
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An Erratum to this article was published on 28 April 2021

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Probiotics are known to protect against liver damage induced by the alcohol and acetaldehyde accumulation associated with alcohol intake. However, there have been few studies of the direct effect of probiotics on alcohol metabolism, and the types of probiotics that were previously analyzed were few in number. Here, we investigated the effects of 19 probiotic species on alcohol and acetaldehyde metabolism. Four probiotic species that had a relatively high tolerance to alcohol and metabolized alcohol and acetaldehyde effectively were identified: Lactobacillus gasseri CBT LGA1, Lactobacillus casei CBT LC5, Bifidobacterium lactis CBT BL3, and Bifidobacterium breve CBT BR3. These species also demonstrated high mRNA expression of alcohol and acetaldehyde dehydrogenases. ProAP4, a mixture of these four probiotics species and excipient, was then administered to rats for 2 weeks in advance of acute alcohol administration. The serum alcohol and acetaldehyde concentrations were significantly lower in the ProAP4-administered group than in the control and excipient groups. Thus, the administration of ProAP4, containing four probiotic species, quickly lowers blood alcohol and acetaldehyde concentrations in an alcohol and acetaldehyde dehydrogenasedependent manner. Furthermore, the serum alanine aminotransferase activity, which is indicative of liver damage, was significantly lower in the ProAP4 group than in the control group. The present findings suggest that ProAP4 may be an effective means of limiting alcohol-induced liver damage.

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  • Ahn, S.B., Jun, D.W., Kang, B.K., Lim, J.H., Lim, S., and Chung, M.J. 2019. Randomized, double-blind, placebo-controlled study of a multispecies probiotic mixture in nonalcoholic fatty liver disease. Sci. Rep. 9, 5688.

    Article  Google Scholar 

  • Ahn, Y.T., Kim, Y.H., Bae, J.S., Lim, K.S., Huh, C.S., Yang, W.Y., Kim, H.S., and Baek, Y.J. 2004. Effect of Lactobacillus brevis HY7401 intake on the serum ethanol concentration in rats. Korean J. Food Sci. Technol. 36, 604–608.

    Google Scholar 

  • Chiang, C.P., Jao, S.W., Lee, S.P., Chen, P.C., Chung, C.C., Lee, S.L., Nieh, S., and Yin, S.J. 2012. Expression pattern, ethanol-metabolizing activities, and cellular localization of alcohol and aldehyde dehydrogenases in human large bowel: association of the functional polymorphisms of ADH and ALDH genes with hemorrhoids and colorectal cancer. Alcohol 46, 37–49.

    Article  CAS  Google Scholar 

  • Chung, W.H., Kang, J., Lim, M.Y., Lim, T.J., Lim, S., Roh, S.W., and Nam, Y.D. 2018. Complete genome sequence and genomic characterization of Lactobacillus acidophilus LA1 (11869BP). Front. Pharmacol. 9, 83.

    Article  Google Scholar 

  • Giannini, E.G., Testa, R., and Savarino, V. 2005. Liver enzyme alteration: a guide for clinicians. CMAJ 172, 367–379.

    Article  Google Scholar 

  • Fuller, F. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66, 365–378.

    Article  CAS  Google Scholar 

  • Goldin, B.R. and Gorbach, S.L. 1984. The effect of milk and lactobacillus feeding on human intestinal bacterial enzyme activity. Am. J. Clin. Nutr. 39, 756–761.

    Article  CAS  Google Scholar 

  • Holt, S. 1981. Observations on the relation between alcohol absorption and the rate of gastric emptying. Can. Med. Assoc. J. 124, 267–277.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Homann, N., Tillonen, J., and Salaspuro, M. 2000. Microbially produced acetaldehyde from ethanol may increase the risk of colon cancer via folate deficiency. Int. J. Cancer 86, 169–173.

    Article  CAS  Google Scholar 

  • Ibáñez, M.D., del Río, P.R., Alsina, D.G.S., and Iglesias, V.V. 2018. Effect of synbiotic supplementation on children with atopic dermatitis: an observational prospective study. Eur. J. Pediatr. 177, 1851–1858.

    Article  Google Scholar 

  • Kang, J., Chung, W.H., Lim, T.J., Lim, S., and Nam, Y.D. 2017a. Complete genome sequence of the Bifidobacterium animalis subspecies lactis BL3, preventive probiotics for acute colitis and colon cancer. New Microbes New Infect. 19, 34–37.

    Article  CAS  Google Scholar 

  • Kang, J., Chung, W.H., Lim, T.J., Whon, T.W., Lim, S., and Nam, Y.D. 2017b. Complete genome sequence of Lactobacillus casei LC5, a potential probiotics for atopic dermatitis. Front. Immunol. 8, 413.

    PubMed  PubMed Central  Google Scholar 

  • Kwak, M.J., Yoon, J.K., Kwon, S.K., Chung, M.J., Seo, J.G., and Kim, J.F. 2015. Complete genome sequence of the probiotic bacterium Bifidobacterium breve KCTC 12201BP isolated from a healthy infant. J. Biotechnol. 214, 156–157.

    Article  CAS  Google Scholar 

  • Koivisto, T. and Salaspuro, M. 1996. Aldehyde dehydrogenases of the rat colon: comparison with other tissues of the alimentary tract and the liver. Alcohol. Clin. Exp. Res. 20, 551–555.

    Article  CAS  Google Scholar 

  • Konkit, M., Kim, K., Kim, J.H., and Kim, W. 2018. Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats. J. Dairy Sci. 101, 5713–5723.

    Article  CAS  Google Scholar 

  • Lee, J., Hwang, B.H., Song, H.J., Jang, S.H., and Choe, S.Y. 2016. Effect of black red ginseng mixture on alcohol metabolism in rats. Korean J. Food Nutr. 29, 655–662.

    Article  Google Scholar 

  • Lieber, C.S. and Decarli, L.M. 1991. Hepatotoxicity of ethanol. J. Hepatol. 12, 394–401.

    Article  CAS  Google Scholar 

  • Morris, R. 1984. Developments of a water-maze procedure for studying spatial learning in the rat. J. Neurosci. Methods 11, 47–60.

    Article  CAS  Google Scholar 

  • Nosova, T., Jokelainen, K., Kaihovaara, P., Jousimies-Somer, H., Siitonen, A., Heine, R., and Salaspuro, M. 1996. Aldehyde dehydrogenase activity and acetate production by aerobic bacteria representing the normal flora of human large intestine. Alcohol Alcohol. 31, 555–564.

    Article  CAS  Google Scholar 

  • Nosova, T., Jousimies-Somer, H., Jokelainen, K., Heine, R., and Salaspuro, M. 2000. Acetaldehyde production and metabolism by human indigenous and probiotic Lactobacillus and Bifidobacterium strains. Alcohol Alcohol. 35, 561–568.

    Article  CAS  Google Scholar 

  • Rossi, M., Jahanzaib Anwar, M., Usman, A., Keshavarzian, A., and Bishehsari, F. 2018. Colorectal cancer and alcohol consumption-populations to molecules. Cancers 10, 38.

    Article  Google Scholar 

  • Salaspuro, M. 1997. Microbial metabolism of ethanol and acetaldehyde and clinical consequences. Addict. Biol. 2, 35–46.

    Article  CAS  Google Scholar 

  • Sung, H.M., Jung, H.J., Yun, S.K., Kim, T.Y., Kim, K.M., and Wee, J.H. 2014. Effect of a soy-sprout beverage prepared with high-concentrated oxygen water on alcohol metabolism in rats. Korean J. Food Sci. Technol. 46, 616–621.

    Article  Google Scholar 

  • Tsuruya, A., Kuwahara, A., Saito, Y., Yamaguchi, H., Tenma, N., Inai, M., Takahashi, S., Tsutsumi, S., Suwa, Y., Totsuka, Y., et al. 2016. Major anaerobic bacteria responsible for the production of carcinogenic acetaldehyde from ethanol in the colon and rectum. Alcohol Alcohol. 51, 395–401.

    Article  CAS  Google Scholar 

  • Vaughn, R.H. 1955. Bacterial spoilage of wines with special reference to California conditions. Adv. Food Res. 6, 67–108.

    Article  CAS  Google Scholar 

  • Visapää, J.P., Tillonen, J., and Salaspuro, M. 2002. Microbes and mucosa in the regulation of intracolonic acetaldehyde concentration during ethanol challenge. Alcohol Alcohol. 37, 322–326.

    Article  Google Scholar 

  • Yang, S.T. 2010. Effects of aged black garlic extract on ethanol induced hangover in rats. J. Life Sci. 20, 225–230.

    Article  Google Scholar 

  • Yim, J.H., Kim, D.H., Ku, J.K., Kang, Y.S., Kim, M.Y., Kim, H.O., Chung, M.J., and Park, Y.M. 2006. Therapeutic effects of probiotics in patients with atopic dermatitis. J. Microbiol. Biotechnol. 16, 1699–1705.

    CAS  Google Scholar 

  • Yin, S.J., Liao, C.S., Lee, Y.C., Wu, C.W., and Jao, S.W. 1994. Genetic polymorphism and activities of human colon alcohol and aldehyde dehydrogenases: no gender and age differences. Alcohol. Clin. Exp. Res. 18, 1256–1260.

    Article  CAS  Google Scholar 

  • Yoon, J.S., Sohn, W., Lee, O.Y., Lee, S.P., Lee, K.N., Jun, D.W., Lee, H.L., Yoon, B.C., Choi, H.S., Chung W.S., et al. 2014. Effect of multispecies probiotics on irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. J. Gastroenterol. Hepatol. 29, 52–59.

    Article  Google Scholar 

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Correspondence to Myung Jun Chung.

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Lim, TJ., Lim, S., Yoon, J.H. et al. Effects of multi-species probiotic supplementation on alcohol metabolism in rats. J Microbiol. 59, 417–425 (2021).

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