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Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system

  • Microbial Ecology and Environmental Microbiology
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Abstract

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named “Fuzi”, which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in short-term continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.

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Acknowledgments

This research was partially supported by the National Science and Technology Project to Benefit the People (Grant number:2012GS610102); Innovation Team Project of Basic Research Fund of Central University(Grant number: GK-202001006); Key Technologies R & D Program of Shaanxi Province (Grant numbers: 2019ZDLSF04-01-01, S2020-YF-YBSF-1029) and National Natural Science Foundation of PR China(Grant numbers: 31801513). We are most grateful to Professor Zhe-Zhi Wang of Shaanxi Normal University for the formal identification of the A. carmichaeli and Professor Q. Fariduddin of Aligarh Muslim University for the manuscript checking. Metagenome sequencing in the current study was performed by Annoroad Gene Technology (Beijing) Co., Ltd.

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Xia F. and Wang L.N. contributed equally to the work. Figure 1A was drawn by Langjun Cui, other pictures were taken by Jiayang Chen.

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Correspondence to Cui Langjun.

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Fei, X., Lina, W., Jiayang, C. et al. Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system. J Microbiol. 59, 481–490 (2021). https://doi.org/10.1007/s12275-021-0515-z

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