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Sulfitobacter profundi sp. nov., isolated from deep seawater

Abstract

A Gram-stain-negative, rod-shaped, obligately aerobic, chemoheterotrophic bacterium which is motile by means of a single polar flagellum, designated SAORIC-263T, was isolated from deep seawater of the Pacific Ocean. Phylogenetic analyses based on 16S rRNA gene sequences and genomebased phylogeny revealed that strain SAORIC-263T belonged to the genus Sulfitobacter and shared 96.1–99.9% 16S rRNA gene sequence similarities with Sulfitobacter species. Wholegenome sequencing of strain SAORIC-263T revealed a genome size of 3.9Mbp and DNA G+C content of 61.3 mol%. The SAORIC-263T genome shared an average nucleotide identity and digital DNA-DNA hybridization of 79.1–88.5% and 18.9–35.0%, respectively, with other Sulfitobacter genomes. The SAORIC-263T genome contained the genes related to benzoate degradation, which are frequently found in deep-sea metagenome. The strain contained summed feature 8 (C18:1ω7c), C18:1ω7c 11-methyl, and C16:0 as the predominant cellular fatty acids as well as ubiquinone-10 (Q-10) as the major respiratory quinone. The major polar lipids of the strain were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, and aminolipid. On the basis of taxonomic data obtained in this study, it is suggested that strain SAORIC-263T represents a novel species of the genus Sulfitobacter, for which the name Sulfitobacter profundi sp. nov. is proposed. The type strain is SAORIC-263T (= KACC 21183T = NBRC 113428T).

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Acknowledgements

We are grateful to the officer and crew of R/V Mirai (Japan Agency for Marine-Earth Science and Technology [JAMSTEC]) for their assistance and support with sample collection. We would also like to thank Dr. Kazuhiro Kogure for his support with the isolation of deep-sea bactrial strains.

This study was supported by Inha University Research Grant.

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Correspondence to Jang-Cheon Cho.

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Song, J., Jang, HJ., Joung, Y. et al. Sulfitobacter profundi sp. nov., isolated from deep seawater. J Microbiol. 57, 661–667 (2019). https://doi.org/10.1007/s12275-019-9150-3

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  • DOI: https://doi.org/10.1007/s12275-019-9150-3

Keywords

  • Sulfitobacter profundi
  • deep sea
  • genome
  • Sulfitobacter
  • Rhodobacteraceae