Paenibacillus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter

  • Soo-Jin Kim
  • Hayoung Cho
  • Jae-Hyung Ahn
  • Hang-Yeon Weon
  • Jae-Ho Joa
  • Jeong-Seon Kim
  • Soon-Wo KwonEmail author


A Gram-stain-positive, rod-shaped, non-endospore-forming, motile by means of peritrichous flagella, facultatively anaerobic bacterium designated TI45-13arT was isolated from Nuruk, a Korean traditional Makgeolli fermentation starter. It grew at 4-35°C (optimum, 28-30°C), pH 5.0-9.0 (optimum, pH 7.0) and NaCl concentrations up to 5% (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain TI45-13arT belonged to the genus Paenibacillus and showed the highest sequence similarities with Paenibacillus kyungheensis DCY88T (98.5%), Paenibacillus hordei RH-N24T (98.4%) and Paenibacillus nicotianae YIM h-19T (98.1%). The major fatty acid was anteiso-C15:0. The DNA G+C content was 39.0 mol%, and MK-7 was the predominant isoprenoid quinone. The polar lipids were diphosphatidylglycerol, phos-phatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids, and one unidentified aminoglycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. On the basis of polyphasic taxonomy study, it was suggested that strain TI45-13arT represents a novel species within the genus Paenibacillus for which the name Paenibacillus nuruki sp. nov. is proposed. The type strain was TI45-13arT (= KACC 18728T = NBRC 112013T).


Korean traditional Makgeolli Paenibacillus nuruki sp. nov. novel species quinone 


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This study was carried out with the support (Project no. PJ-011248) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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Copyright information

© The Microbiological Society of Korea 2019

Authors and Affiliations

  • Soo-Jin Kim
    • 1
  • Hayoung Cho
    • 1
  • Jae-Hyung Ahn
    • 1
  • Hang-Yeon Weon
    • 1
  • Jae-Ho Joa
    • 2
  • Jeong-Seon Kim
    • 1
  • Soon-Wo Kwon
    • 1
    Email author
  1. 1.Rural Development AdministrationAgricultural Microbiology Division, National Institute of Agricultural SciencesWanjuRepublic of Korea
  2. 2.Rural Development AdministrationNational Institute of Horticultural and Herbal ScienceJejuRepublic of Korea

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