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Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated YLB-03T, with peritrichous flagella was isolated from deep-sea sediment of the Yap Trench at a depth of 4435 m. The bacterium was found to be catalase-positive but oxidase-negative. Growth of this bacterium was observed at 15–50°C (optimum 37°C), pH 5–10.5 (optimum 7), 0–5% NaCl (optimum 1%, w/v) and 0.1–50 MPa (optimum 0.1 MPa). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YLB-03T was a member of the genus Lysinibacillus. Strain YLB-03T was closely related to Lysinibacillus sinduriensis BLB-1T and Lysinibacillus chungkukjangi 2RL3-2T (98.4%), Lysinibacillus halotolerans LAM-612T (98.0%), Lysinibacillus telephonicus KT735049T (97.5%), Lysinibacillus endophyticus C9T (97.5%), Lysinibacillus composti NCCP-36T and Lysinibacillus massiliensis 4400831T (97.3%). The ANI and the GGDC DNA-DNA hybridization estimate values between strain YLB-03T and closely related type strains were 73.7–76.3% and 34.7–38.7%, respectively. The principal fatty acids were anteiso-C15:0 and iso-C15:0. The G+C content of the chromosomal DNA was 39.6 mol%. The respiratory quinone was determined to be MK-7. The diagnostic amino acids in the cell wall peptidoglycan contained Lys-Asp (type A4α) and the cell-wall sugars were glucose and xylose. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The combined genotypic and phenotypic data showed that strain YLB-03T represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus yapensis sp. nov. is proposed, with the type strain YLB-03T (= MCCC 1A12698T = JCM 32871T).

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Acknowledgements

This work was supported by the National Basic Research Program of China (973 Program) (No.2015CB755901), COMRA Project of China (DY135-B2-16), Fujian Key Science and Technology Program (No.2018N0017), Xiamen Ocean Economic Innovation and Development Demonstration Project (No.16PZP001SF16), Scientific Research Foundation of Third Institute of Oceanography, SOA. (No.2016002, 2017002), Xiamen Science and Technology Program (No. 3502Z2018-2029), and Ministry of Natural Resources supporting project of Marine Biodiversity of Areas National Jurisdiction, No. HC180701. We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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Authors and Affiliations

  1. School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, P. R. China

    Libo Yu & Qian Yang

  2. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, P. R. China

    Libo Yu, Xixiang Tang, Xiashutong Xu, Guangxin Xu & Qilin Wang

  3. School of Ocean Sciences, China University of Geosciences, Beijing, 100083, P. R. China

    Shiping Wei, Xiashutong Xu, Guangxin Xu & Qilin Wang

  4. Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361102, P. R. China

    Yinkun Qiu

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Yu, L., Tang, X., Wei, S. et al. Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean. J Microbiol. 57, 562–568 (2019). https://doi.org/10.1007/s12275-019-8709-3

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