Abstract
Two Gram-stain negative halophilic strains, designated as LM2T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase-positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2T and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C18:1ω7c/C18:1ω6c, C16:0, C19:0 cyclo ω8c, and 11-methyl C18:1ω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2T and LM4, 24.9% relatedness between LM2T and R. tolerans EL-172T, and 36.3% relatedness between LM4 and R. tolerans EL-172T. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2T (= CGMCC 1.16230T = KCTC 62028T).
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Achenbach, L.A., Carey, J., and Madigan, M.T. 2001. Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments. Appl. Environ. Microbiol. 67, 2922–2926.
Allgaier, M., Uphoff, H., Felske, A., and Wagner-Dobler, I. 2003. Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl. Environ. Microbiol. 69, 5051–5059.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403–410.
De Ley, J., Cattoir, H., and Reynaerts, A. 1970. The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12, 133–142.
Dong, X.Z. and Cai, M.Y. 2001. Determinative manual for routine bacteriology. Beijing Scientific Press, Beijing, China.
Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.
Jung, Y.T., Lee, J.S., Oh, K.H., Oh, T.K., and Yoon, J.H. 2011. Roseovarius marinus sp. nov., isolated from seawater. Int. J. Syst. Evol. Microbiol. 61, 427–432.
Kimura, M. 1979. The neutral theory of molecular evolution. Sci. Am. 241, 98–100, 102, 108.
Kluge, A.G. and Farris, J.S. 1969. Quantitative phyletics and the evolution of Anurans. Syst. Biol. 18, 1–32.
Kuykendall, L.D., Roy, M.A., O’Neill, J.J., and Devine, T.E. 1988. Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int. J. Syst. Bacteriol. 38, 358–361.
Labrenz, M., Collins, M.D., Lawson, P.A., Tindall, B.J., Schumann, P., and Hirsch, P. 1999. Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int. J. Syst. Bacteriol. 49, 137–147.
Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115–175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid sequencing techniques in bacterial systematics, Wiley, New York, USA.
Li, Z., Zhao, R., Ji, S., Shi, X., and Zhang, X.H. 2013. Roseovarius marisflavi sp. nov., isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China. Antonie van Leeuwenhoek 104, 413–421.
Marmur, J. and Doty, P. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5, 109–118.
Martens, T., Heidorn, T., Pukall, R., Simon, M., Tindall, B.J., and Brinkhoff, T. 2006. Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int. J. Syst. Evol. Microbiol. 56, 1293–1304.
Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.
Oh, Y.S., Lim, H.J., Cha, I.T., Im, W.T., Yoo, J.S., Kang, U.G., Rhee, S.K., and Roh, D.H. 2009. Roseovarius halotolerans sp. nov., isolated from deep seawater. Int. J. Syst. Evol. Microbiol. 59, 2718–2723.
Pujalte, M., Lucena, T., Ruvira, M., Arahal, D., and Macián, M. 2014. The Family Rhodobacteraceae. Springer, Heidelberg, Berlin, Germany.
Rajasabapathy, R., Mohandass, C., Dastager, S.G., Liu, Q., Khieu, T.N., Son, C.K., Li, W.J., and Colaco, A. 2014. Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores. Antonie van Leeuwenhoek 105, 571–578.
Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Sasser, M. 1990. Identification of bacteria through fatty acid analysis, pp. 199–204. In Klement, Z., Rudolph, K., and Sands, D.C. (eds.), Methods in phytobacteriology, Akademiai Kaido, Budapest, Hungary.
Shiba, T., Simidu, U., and Taga, N. 1979. Distribution of aerobic bacteria which contain bacteriochlorophyll a. Appl. Environ. Microbiol. 38, 43–45.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729.
Tang, X., Zhai, L., Lin, Y., Yao, S., Wang, L., Ge, Y., Liu, Y., Zhang, X., Zhang, T., Zhang, L., et al. 2017. Halomonas alkalicola sp. nov., isolated from a household product plant. Int. J. Syst. Evol. Microbiol. 67, 1546–1550.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.
Tindall, B.J. 1990. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol. Lett. 66, 199–202.
Vannini, C., Petroni, G., Verni, F., and Rosati, G. 2005. A bacterium belonging to the Rickettsiaceae family inhabits the cytoplasm of the marine ciliate Diophrys appendiculata (Ciliophora, Hypotrichia). Microb. Ecol. 49, 434–442.
Wang, B., Sun, F., Lai, Q., Du, Y., Liu, X., Li, G., Luo, J., and Shao, Z. 2010. Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment. Int. J. Syst. Evol. Microbiol. 60, 1289–1295.
Wang, B., Tan, T., and Shao, Z. 2009. Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int. J. Syst. Evol. Microbiol. 59, 1116–1121.
Wayne, L.G., Brenner, D.J., Colwell, R.R., Grimont, P.A.D., Kandler, O., Krichevsky, M.I., Moore, L.H., Moore, W.E.C., Murry, R.G.E., Stackebrandt, E., et al. 1987. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.
Yoon, S.H., Ha, S.M., Kwon, S., Lim, J., Kim, Y., Seo, H., and Chun, J. 2016. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int. J. Syst. Evol. Microbiol. 67, 1613–1617.
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Lu, Hb., Xue, Xf., Phurbu, D. et al. Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau. J Microbiol. 56, 783–789 (2018). https://doi.org/10.1007/s12275-018-8178-0
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DOI: https://doi.org/10.1007/s12275-018-8178-0