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Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage

  • Microbial Physiology and Biochemistry
  • Published:
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Abstract

Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. School of Food Science and Engineering, Hefei University of Technology, Hefei, P. R. China

    Liu-Hui Fu, Zeng-Zheng Wei, Kang-Di Hu, Lan-Ying Hu, Yan-Hong Li, Xiao-Yan Chen, Zhuo Han, Gai-Fang Yao & Hua Zhang

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  1. Liu-Hui Fu
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Correspondence to Gai-Fang Yao or Hua Zhang.

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Fu, LH., Wei, ZZ., Hu, KD. et al. Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage. J Microbiol. 56, 238–245 (2018). https://doi.org/10.1007/s12275-018-7537-1

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