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Mucilaginibacter hankyongensis sp. nov., isolated from soil of ginseng field Baekdu Mountain

Journal of Microbiology volume 55, pages 525–530 (2017)Cite this article

Abstract

A Gram-negative, non-motile, aerobic, and rod-shaped bacterial strain designated as BR5-28T was isolated from the soil of a ginseng field at Baekdu Mountain Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BR5-28T grew at 10–42°C (optimum temperature, 30°C) and pH 5.5–8.5 (optimum pH, 7.0) on R2A agar medium without additional NaCl supplementation. Strain BR5- 28T exhibited β-glucosidase activity, which was responsible for its ability to transform the ginsenosides Rb1 and Rd (the two dominant active components of ginseng) to compound-K. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Mucilaginibacter of the family Sphingobacteriaceae, and formed clusters with Mucilaginibacter frigoritolerans FT22T (95.8%) and Mucilaginibacter gotjawali SA3-7T (95.7%). The G+C content of the genomic DNA was 45.1%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c), iso-C15:0 and anteiso-C15:0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain BR5-28T was differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter hankyongensis sp. nov. is proposed, with the type strain BR5-28T (=KCTC 22274T =DSM 21151T).

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Authors and Affiliations

  1. Department of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea

    Qingmei Liu, Muhammad Zubair Siddiqi & Wan-Taek Im

  2. Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Anseong, 17579, Republic of Korea

    Qingmei Liu, Muhammad Zubair Siddiqi & Wan-Taek Im

  3. Clean Fuel Research Center, Korea Institute of Energy Research, Daejeon, 34129, Republic of Korea

    Mi-Sun Kim

  4. Department of Food Science & Bio Technology, Shinansan University, Ansan, 15435, Republic of Korea

    Sang Yong Kim

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  1. Qingmei Liu
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  2. Muhammad Zubair Siddiqi
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Correspondence to Wan-Taek Im.

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Liu, Q., Siddiqi, M.Z., Kim, MS. et al. Mucilaginibacter hankyongensis sp. nov., isolated from soil of ginseng field Baekdu Mountain. J Microbiol. 55, 525–530 (2017). https://doi.org/10.1007/s12275-017-7180-2

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  • DOI: https://doi.org/10.1007/s12275-017-7180-2

Keywords

  • 16S rRNA gene
  • polyphasic taxonomy
  • Mucilaginibacter hankyongensis
  • ginsenoside