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Alteration in the ultrastructural morphology of mycelial hyphae and the dynamics of transcriptional activity of lytic enzyme genes during basidiomycete morphogenesis

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

The morphogenesis of macromycetes is a complex multilevel process resulting in a set of molecular-genetic, physiological-biochemical, and morphological-ultrastructural changes in the cells. When the xylotrophic basidiomycetes Lentinus edodes, Grifola frondosa, and Ganoderma lucidum were grown on wood waste as the substrate, the ultrastructural morphology of the mycelial hyphal cell walls differed considerably between mycelium and morphostructures. As the macromycetes passed from vegetative to generative development, the expression of the tyr1, tyr2, chi1, chi2, exg1, exg2, and exg3 genes was activated. These genes encode enzymes such as tyrosinase, chitinase, and glucanase, which play essential roles in cell wall growth and morphogenesis.

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Authors and Affiliations

  1. Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences IBPPM RAS, 13 Prospekt Entuziastov, Saratov, 410049, Russian Federation

    Elena Vetchinkina, Maria Kupryashina & Valentina Nikitina

  2. Laboratory of Molecular Biology, Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, 2/31 Lobachevsky street, Kazan, 420111, Russian Federation

    Vladimir Gorshkov, Marina Ageeva & Yuri Gogolev

  3. Kazan Federal University, 18 Kremlyovskaya street, Kazan, 420008, Russian Federation

    Vladimir Gorshkov & Yuri Gogolev

Authors
  1. Elena Vetchinkina
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  2. Maria Kupryashina
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  3. Vladimir Gorshkov
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  4. Marina Ageeva
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  5. Yuri Gogolev
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  6. Valentina Nikitina
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Correspondence to Elena Vetchinkina.

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Vetchinkina, E., Kupryashina, M., Gorshkov, V. et al. Alteration in the ultrastructural morphology of mycelial hyphae and the dynamics of transcriptional activity of lytic enzyme genes during basidiomycete morphogenesis. J Microbiol. 55, 280–288 (2017). https://doi.org/10.1007/s12275-017-6320-z

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  • DOI: https://doi.org/10.1007/s12275-017-6320-z

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