Abstract
A Gram-staining-negative, aerobic, non-flagellated, rod-shaped bacterial strain, KIS55-21T, was isolated from a soil sample from Chuja Island, Jeju Province, Republic of Korea. Strain KIS55-21T grew optimally at pH 7.0, at 28–30°C and in the presence of 0% (w/v) NaCl. Neighbor-joining and maximum-likelihood trees based on the 16S rRNA gene sequences revealed that strain KIS55-21T fell within the family Xanthomonadaceae and was closely related to Metallibacterium scheffleri DKET. Strain KIS55-21T exhibited the highest 16S rRNA gene sequence similarity (92.6%) to that of M. scheffleri DKET, with similarities of less than 92.0% to those of the genera Dokdonella, Rhodanobacter, Aquimonas, and Frateuria. Strain KIS55-21T contained ubiquinone-8 (Q-8) as the predominant ubiquinone, iso-C17:0, summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl), anteiso-C17:0 and C16:0 as the major fatty acids, and phosphatidylethanolamine, aminophospholipid, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylmonomethylethanolamine as the main polar lipids. The DNA G+C content of strain KIS55-21T was 65.9 mol%. Differential phenotypic and chemotaxonomic properties and phylogenetic data of strain KIS55-21T demonstrated that this strain is distinguishable from closely related genera within the family Xanthomonadaceae. On the basis of the data presented, strain KIS55-21T is considered to represent a novel genus and species, for which the name Chujaibacter soli gen. nov., sp. nov. is proposed. The type strain is KIS55-21T (=KACC 16971T =DSM 28578T).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Anandham, R., Kwon, S.W., Indira Gandhi, P., Kim, S.J., Weon, H.Y., Kim, Y.S., Sa, T.M., Kim, Y.K., and Jee, H.J. 2011. Dyella thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate- oxidizing bacterium isolated from rhizosphere soil of sunflower (Helianthus annuus L.). Int. J. Syst. Evol. Microbiol. 61, 392–398.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. 1987. Current Protocols in Molecular Biology. Greene/Wiley Interscience, New York, N.Y., USA.
Breznak, J.A. and Costilow, R.N. 1994. Physicochemical factors in growth, pp. 137–154. In Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. (eds.), Methods for General and Molecular Bacteriology, American Society for Microbiology, Washington, D.C., USA.
Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376.
Gonzalez, J.M. and Saiz-Jimenez, C. 2002. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770–773.
Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716–721.
Kluge, A.G. and Farris, J.S. 1969. Quantitative phyletics and the evolution of anurans. Syst. Zool. 18, 1–32.
Mergaert, J., Cnockaert, M.C., and Swings, J. 2002. Fulvimonas soli gen. nov. sp. nov., a γ-proteobacterium isolated from soil after enrichment on acetylated starch plastic. Int. J. Syst. Evol. Microbiol. 52, 1285–1289.
Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.
Moreira, A.P.B., Pereira Jr., N., and Thompson, F.L. 2011. Usefulness of a real-time PCR platform for G+C content and DNA-DNA hybridization estimations in Vibrios. Int. J. Syst. Evol. Microbiol. 61, 2379–2383.
Nalin, R., Simonet, P., Vogel, T.M., and Normand, P. 1999. Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. Int. J. Syst. Bacteriol. 49, 19–23.
Pruesse, E., Peplies, J., and Glöckner, F.O. 2012. SINA: accurate highthroughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28, 1823–1829.
Saddler, G.S. and Bradbury, J.F. 2005. Order III. Xanthomonadales ord. nov. p. 63. In Brenner, D.J., Krieg, N.R., Staley, J.T., and Garrity, G.M. (eds.), Bergey’s Manual of Systematic Bacteriology, vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria) 2nd ed., Springer, New York, USA.
Saha, P., Krishnamurthi, S., Mayilraj, S., Prasad, G.S., Bora, T.C., and Chakrabarti, T. 2005. Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int. J. Syst. Evol. Microbiol. 55, 1491–1495.
Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc., Newark, DE, USA.
Swings, J., Gillis, M., Kersters, K., Gossele, F., and De Ley, J. 1980. Frateuria, a new genus for “Acetobacter aurantius”. Int. J. Syst. Bacteriol. 30, 547–556.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.
Ten, L.N., Jung, H.M., Im, W.T., Oh, H.W., Yang, D.C., Yoo, S.A., and Lee, S.T. 2009. Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella. Int. J. Syst. Evol. Microbiol. 59, 1947–1952.
Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.
Weon, H.Y., Yoo, S.H., Kim, Y.J., Lee, C.M., Kim, B.Y., Jeon, Ya., Hong, S.B., Anandham, R., and Kwon, S.W. 2009. Rudaea cellulosilytica gen. nov., sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 59, 2308–2312.
Woo, S.G., Srinivasan, S., Kim, M.K., and Lee, M. 2012. Rhodanobacter caeni sp. nov., isolated from sludge from a sewage disposal plant. Int. J. Syst. Evol. Microbiol. 62, 2815–2821.
Yoon, J.H., Kang, S.J., and Oh, T.K. 2006. Dokdonella koreensis gen. nov., sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 56, 145–150.
Zhang, J.Y., Liu, X.Y., and Liu, S.J. 2011. Frateuria terrea sp. nov., isolated from forest soil, and emended description of the genus Frateuria. Int. J. Syst. Evol. Microbiol. 61, 443–447.
Ziegler, S., Waidner, B., Itoh, T., Schumann, P., Spring, S., and Gesche, J. 2013. Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm. Int. J. Syst. Evol. Microbiol. 63, 1499–1504.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supplemental material for this article may be found at http://www.springerlink.com/content/120956.
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Kim, SJ., Ahn, JH., Weon, HY. et al. Chujaibacter soli gen. nov., sp. nov., isolated from soil. J Microbiol. 53, 592–597 (2015). https://doi.org/10.1007/s12275-015-5136-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-015-5136-y