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Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Journal of Microbiology volume 53, pages 1–5 (2015)Cite this article

Abstract

Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification. In the LAMP reaction, samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions: self-elongation of templates from the stem loop structure formed at the 3′-terminal and the binding and elongation of new primers to the loop region. The LAMP reaction has a wide range of possible applications, including point-of-care testing, genetic testing in resource-poor settings (such as in developing countries), and rapid testing of food products and environmental samples.

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Affiliations

  1. Biochemical Research Laboratory, Research & Development Division, Eiken Chemical Co., Ltd. 1381-3 Shimoishigami, Ohtawara, Tochigi, 324-0036, Japan

    Tsugunori Notomi, Yasuyoshi Mori, Norihiro Tomita & Hidetoshi Kanda

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  1. Tsugunori Notomi
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  2. Yasuyoshi Mori
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  3. Norihiro Tomita
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  4. Hidetoshi Kanda
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Correspondence to Tsugunori Notomi.

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Notomi, T., Mori, Y., Tomita, N. et al. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J Microbiol. 53, 1–5 (2015). https://doi.org/10.1007/s12275-015-4656-9

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  • DOI: https://doi.org/10.1007/s12275-015-4656-9

Keywords

  • loop-mediated isothermal amplification (LAMP)
  • nucleic acid amplification test (NAT)
  • point of care testing (POCT)