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A potent Brucella abortus 2308 Δery live vaccine allows for the differentiation between natural and vaccinated infection

  • Microbial Pathogenesis and Host-Microbe Interaction
  • Published:
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Abstract

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

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Authors and Affiliations

  1. College of Animal Science and Technology, Shihezi University, Shihezi, 832003, P. R. China

    Junbo Zhang, Ren Meng, Chuangfu Chen, Hui Zhang, Zhiqiang Li, Qiang Fu, Huijun Shi, Shengwei Hu, Tiansen Li & Ke Zhang

  2. College of Medicine, Shihezi University, Shihezi, 832003, P. R. China

    Shuanghong Yin & Fei Guo

  3. College of Life Sciences, Shihezi University, Xinjiang, 832000, P. R. China

    Wei Ni

Authors
  1. Junbo Zhang
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  2. Shuanghong Yin
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  3. Fei Guo
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  4. Ren Meng
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  5. Chuangfu Chen
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  6. Hui Zhang
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  7. Zhiqiang Li
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  8. Qiang Fu
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  9. Huijun Shi
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  10. Shengwei Hu
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  11. Wei Ni
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  12. Tiansen Li
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  13. Ke Zhang
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Correspondence to Chuangfu Chen.

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These authors contributed equally to the work.

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Zhang, J., Yin, S., Guo, F. et al. A potent Brucella abortus 2308 Δery live vaccine allows for the differentiation between natural and vaccinated infection. J Microbiol. 52, 681–688 (2014). https://doi.org/10.1007/s12275-014-3689-9

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  • DOI: https://doi.org/10.1007/s12275-014-3689-9

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