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Assessment of conjugal transfer of antibiotic resistance genes in Salmonella Typhimurium exposed to bile salts

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Abstract

This study was designed to evaluate the transfer potential of antibiotic resistance genes in antibiotic-resistant Salmonella Typhimurium (S. TyphimuriumR) in the presence of bile salts. The resistance of S. TyphimuriumR to ampicillin, kanamycin, and tetracycline was increased by 64-, 64-, and 512-fold, respectively. The highest transfer frequency from S. TyphimuriumR to Escherichia coli was observed at the bile salt concentration of 160 μg/ml (3.8 × 10−3 transferrants/cells). The expression of traJ and traY was suppressed in S. TyphimuriumR by bile salt. This study provides useful information for understanding the conjugative transfer of antibiotic resistance genes in S. Typhimurium under intestinal conditions.

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  1. Xinlong He

    Present address: Institute of Burn Research, The Third People’s Hospital, Wuxi, Jiangsu, 214041, P. R. China

Authors and Affiliations

  1. Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Xinlong He & Juhee Ahn

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  1. Xinlong He
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  2. Juhee Ahn
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Correspondence to Juhee Ahn.

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He, X., Ahn, J. Assessment of conjugal transfer of antibiotic resistance genes in Salmonella Typhimurium exposed to bile salts. J Microbiol. 52, 716–719 (2014). https://doi.org/10.1007/s12275-014-3340-9

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  • DOI: https://doi.org/10.1007/s12275-014-3340-9

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