Streptococcus mutans is associated with dental caries. A cariogenic biofilm, in particular, has been studied extensively for its role in the formation of dental caries. Herbal extracts such as Cudrania tricuspidata, Sophora flavescens, Ginkgo biloba, and Betula Schmidtii have been used as a folk remedy for treating diseases. The purpose of this study was to evaluate and compare the antibacterial activity of herbal extracts against normal oral streptococci, planktonic and biofilm of S. mutans. Streptococcus gordonii, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguinis, and S. mutans were cultivated with brain heart infusion broth and susceptibility assay for the herbal extracts was performed according to the protocol of Clinical and Laboratory Standard Institute. Also, S. mutans biofilm was formed on a polystyrene 12-well plate and 8-well chamber glass slip using BHI broth containing 2% sucrose and 1% mannose after conditioning the plate and the glass slip with unstimulated saliva. The biofilm was treated with the herbal extracts in various concentrations and inoculated on Mitis-Salivarius bacitracin agar plate for enumeration of viable S. mutans by counting colony forming units. Planktonic S. mutans showed susceptibility to all of the extracts and S. mutans biofilm exhibited the highest level of sensitivity for the extracts of S. flavescens. The normal oral streptococci exhibited a weak susceptibility in comparison to S. mutans. S. oralis, however, was resistant to all of the extracts. In conclusion, the extract of S. flavescens may be a potential candidate for prevention and management of dental caries.
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Aoki, H., Shiroza, T., Hayakawa, M., Sato, S., and Kuramitsu, H. K. 1986. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect. Immun. 53, 587–594.
Baek, D.H. 2007. Screening of antimicrobial activity among the therapeutic herbal extracts on dental pathogens. Int’l J. Oral Biol. 32, 75–78.
Bajpai, V.K., Sharma, A., and Baek, K.H. 2013. Antibacterial mode of action of cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens. Food Control 32, 582–590.
Cheung, Z.H., So, K.F., Lu, Q., Yip, H.K., Wu, W., Shan, J.J., Pang, P.K., and Chen, C.F. 2002. Enhanced survival and regeneration of axotomized retinal ganglion cells by a mixture of herbal extracts. J. Neurotrauma 19, 369–378.
Clinical and Laboratory Standards Institute. 2012. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard 8th edition M11-A8, PA, USA.
Costerton, J.W. 1999. Introduction to biofilm. Int. J. Antimicrob. Agents 11, 217–221; discussion 237–219.
Gilbert, P. and Allison, D.G. 1999. Biofilms and their resistance towards antimicrobial agents. Bioline 125–143.
Haffajee, A.D. and Socransky, S.S. 2006. Introduction to microbial aspects of periodontal biofilm communities, development and treatment. Periodontol 2000 42, 7–12.
Hamilton, I.R. and Buckley, N.D. 1991. Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol. Immunol. 6, 65–71.
Han, C. and Guo, J. 2012. Antibacterial and anti-inflammatory activity of traditional chinese herb pairs, Angelica sinensis and Sophora flavescens. Inflammation 35, 913–919.
Hanada, N. and Kuramitsu, H.K. 1988. Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans. Infect. Immun. 56, 1999–2005.
Hanada, N. and Kuramitsu, H.K. 1989. Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer-dependent soluble glucan synthesis. Infect. Immun. 57, 2079–2085.
Joo, H.Y. and Lim, K.T. 2009. Glycoprotein isolated from Cudrania tricuspidata Bureau inhibits iNO and COX-2 expression through modulation of NF-κB in LPS-stimulated raw 264.7 cells. Environ. Toxicol. Pharmacol. 27, 247–252.
Kim, C.S., Park, S.N., Ahn, S.J., Seo, Y.W., Lee, Y.J., Lim, Y.K., Freire, M.O., Cho, E., and Kook, J.K. 2013. Antimicrobial effect of sophoraflavanone G isolated from Sophora flavescens against mutans streptococci. Anaerobe 19, 17–21.
Kotakadi, V.S., Jin, Y., Hofseth, A.B., Ying, L., Cui, X., Volate, S., Chumanevich, A., Wood, P.A., Price, R.L., McNeal, A., and et al. 2008. Ginkgo biloba extract EGb 761 has anti-inflammatory properties and ameliorates colitis in mice by driving effector T cell apoptosis. Carcinogenesis 29, 1799–1806.
Lee, S.H., Choi, B.K., and Kim, Y.J. 2012. The cariogenic characters of xylitol-resistant and xylitol-sensitive Streptococcus mutans in biofilm formation with salivary bacteria. Arch. Oral Biol. 57, 697–703.
Lee, I.K., Kim, C.J., Song, K.S., Kim, H.M., Koshino, H., Uramoto, M., and Yoo, I.D. 1996. Cytotoxic benzyl dihydroflavonols from cudrania tricuspidata. Phytochemistry 41, 213–216.
Loesche, W.J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353–380.
Marsh, P.D. and Bradshaw, D.J. 1995. Dental plaque as a biofilm. J. Ind. Microbiol. 15, 169–175.
Monchois, V., Willemot, R.M., and Monsan, P. 1999. Glucansucrases: Mechanism of action and structure-function relationships. FEMS Microbiol. Rev. 23, 131–151.
Pasquantonio, G., Greco, C., Prenna, M., Ripa, C., Vitali, L.A., Petrelli, D., Di Luca, M.C., and Ripa, S. 2008. Antibacterial activity and anti-biofilm effect of chitosan against strains of Streptococcus mutans isolated in dental plaque. Int. J. Immunopathol. Pharmacol. 21, 993–997.
Sati, S.C. and Joshi, S. 2011. Antibacterial activities of Ginkgo biloba l. Leaf extracts. ScientificWorldJournal 11, 2241–2246.
Sbordone, L. and Bortolaia, C. 2003. Oral microbial biofilms and plaque-related diseases: Microbial communities and their role in the shift from oral health to disease. Clin. Oral Investig. 7, 181–188.
Sendamangalam, V., Choi, O.K., Kim, D., and Seo, Y. 2011. The anti-biofouling effect of polyphenols against Streptococcus mutans. Biofouling 27, 13–19.
Socransky, S.S. and Haffajee, A.D. 2002. Dental biofilms: Difficult therapeutic targets. Periodontol 2000. 28, 12–55.
Vellas, B., Coley, N., Ousset, P.J., Berrut, G., Dartigues, J.F., Dubois, B., Grandjean, H., Pasquier, F., Piette, F., Robert, P., and et al. 2012. Long-term use of standardised Ginkgo biloba extract for the prevention of Alzheimer’s disease (GuidAge): A randomized placebo-controlled trial. Lancet Neurol. 11, 851–859.
Zhang, S., Chen, B., Wu, W., Bao, L., and Qi, R. 2011. Ginkgolide b reduces inflammatory protein expression in oxidized low-density lipoprotein-stimulated human vascular endothelial cells. J. Cardiovasc. Pharmacol. 57, 721–727.
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Lee, SH. Antimicrobial effects of herbal extracts on Streptococcus mutans and normal oral streptococci. J Microbiol. 51, 484–489 (2013). https://doi.org/10.1007/s12275-013-3312-5
- antibacterial activity
- herbal extracts
- S. mutans biofilm