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Lactobacillus paracasei subsp. paracasei LC01 positively modulates intestinal microflora in healthy young adults

  • Microbial Ecology and Environmental Microbiology
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Abstract

Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 1010 CFU of LC01 each day for the 4-week treatment period, and then consumed placebo in the next treatment period, separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichiacoli and increase in Lactobacillus, Bifidobacterium, and Roseburiaintestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.

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Authors and Affiliations

  1. Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P. R. China

    Hao Zhang, Jing Sun, Xianting Liu, Chuan Hong, Yuanbo Zhu, Siqi Li, Huiyuan Guo & Fazheng Ren

  2. Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, P. R. China

    Hao Zhang & Fazheng Ren

  3. Diary Department, MengNiu Diary Company, Beijing, P. R. China

    Aiping Liu

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  1. Hao Zhang
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  2. Jing Sun
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  3. Xianting Liu
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  4. Chuan Hong
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  5. Yuanbo Zhu
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  6. Aiping Liu
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  7. Siqi Li
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  8. Huiyuan Guo
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  9. Fazheng Ren
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Correspondence to Fazheng Ren.

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These authors contributed equally to this work.

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Zhang, H., Sun, J., Liu, X. et al. Lactobacillus paracasei subsp. paracasei LC01 positively modulates intestinal microflora in healthy young adults. J Microbiol. 51, 777–782 (2013). https://doi.org/10.1007/s12275-013-3279-2

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  • DOI: https://doi.org/10.1007/s12275-013-3279-2

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