A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, non-endospore-forming and grew with 0–8.5% (w/v) NaCl, at 15–48°C and at pH 6.5–9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98T forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA-DNA relatedness values of strain KJW98T with B. beijingensis DSM 19037T, B. cecembensis LMG 24411T and B. ginsengi DSM 19038T were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 (37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW98T should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98T (=KCTC 13583T =LMG 25219T).
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Altschul, S.F., Madden, T.L., Schaeffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res.25, 3389–3402.
Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B.K., and Lim, Y.W. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol.57, 2259–2261.
Collins, M.D., Pirouz, T., Goodfellow, M., and Minnikin, D.E. 1977. Distribution of menaquinones in Actinomycetes and Corynebacteria. J. Gen. Microbiol.100, 221–230.
De Ley, J., Cattoir, H., and Reynaerts, A. 1970. The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem.12, 133–142.
Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool.20, 406–416.
Groth, I., Schumann, P., Weiss, N., Martin, K., and Rainey, F.A. 1996. Agrococcus jenensis gen. nov., sp. nov., a new genus of Actinomycetes with diaminobutyric acid in the cell wall. Int. J. Syst. Bacteriol.46, 234–239.
Hauben, L., Vauterin, L., Swings, J., and Moore, E.R.B. 1997. Comparison of 16S ribosomal DNA sequences of all Xanthomonas species. Int. J. Syst. Bacteriol.47, 328–335.
Huss, V.A.R., Festl, H., and Schleifer, K.H. 1983. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol.4, 184–192.
Kämpfer, P., Rossello-Mora, R., Falsen, E., Busse, H.J., and Tindall, B.J. 2006. Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int. J. Syst. Evol. Microbiol.56, 781–786.
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16, 111–120.
Kovacs, N. 1956. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature178, 703.
Logan, N.A., Berge, O., Bishop, A.H., Busse, H.-J., De Vos, P., Fritze, D., Heyndrickx, M., Kämpfer, P., Rabinovitch, L., Salkinoja-Salonen, M.S., andet al. 2009. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int. J. Syst. Evol. Microbiol.59, 2114–2121.
MacKenzie, S.L. 1987. Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J. Assoc. Off. Anal. Chem.70, 151–160.
Manorama, R., Pindi, P.K., Reddy, G.S.N., and Shivaji, S. 2009. Bhargavaea cecembensis gen. nov., sp. nov., isolated from the Chagos-Laccadive ridge system in the Indian Ocean. Int. J. Syst. Evol. Microbiol.59, 2618–2623.
Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int. J. Syst. Bacteriol.39, 159–167.
Pike, E.B., Carringtoen, E.G., and Ashburner, A.P. 1972. An evaluation of procedures for enumerating bacteria in activated sludge. J. Appl. Bacteriol.35, 309–321.
Qiu, F., Zhang, X., Liu, L., Sun, L., Schumann, P., and Song, W. 2009. Bacillus beijingensis sp. nov. and Bacillus ginsengi sp. nov., isolated from ginseng root. Int. J. Syst. Evol. Microbiol.59, 729–734.
Rzhetsky, A. and Nei, M. 1992. A simple method for estimating and testing minimum evolution trees. Mol. Biol. Evol.9, 945–967.
Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4, 406–425.
Schleifer, K.H. 1985. Analysis of the chemical composition and primary structure of murein. Methods Microbiol.18, 123–156.
Schleifer, K.H. and Kandler, O. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev.36, 407–477.
Tamaoka, J. and Komagata, K. 1984. Determination of DNA base composition by rever-sed-phase high-performance liquid chromatography. FEMS Microbiol. Lett.25, 125–128.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol.28, 2731–2739.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res.25, 4876–4882.
Tindall, B.J. 1990a. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst. Appl. Microbiol.13, 128–130.
Tindall, B.J. 1990b. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol. Lett.66, 199–202.
Verma, P., Pandey, P.K., Gupta, A.K., Seong, C.N., Park, S.C., Choe, H.N., Baik, K.S., Patole, M.S., and Shouche, Y.S. 2012. Reclassification of Bacillus beijingensis and Bacillus ginsengi Qiu et al., 2009 as Bhargavaea beijingensis comb. nov. and Bhargavaea ginsengi comb. nov. and emended description of the genus Bhargavaea. Int. J. Syst. Evol. Microbiol.62, 2495–2504.
Wayne, L.G., Brenner, D.J., Colwell, R.R., Grimont, P.A.D., Kandler, O., Krichevsky, M.I., Moore, L.H., Moore, W.E.C., Murray, R.G.E., Stackebrandt, E., andet al. 1987. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol.37, 463–464.
Xia, X. and Xie, Z. 2001. DAMBE: Data analysis in molecular biology and evolution. J. Hered.92, 371–373.
Yamamoto, S. and Harayama, S. 1995. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl. Environ. Microbiol.61, 1104–1109.
Yamamoto, S. and Harayama, S. 1998. Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. Int. J. Syst. Bacteriol.48, 813–819.
Zhang, Z., Schwartz, S., Wagner, L., and Miller, W. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol.7, 203–214.
Zuckerkandl, E. and Pauling, L. 1965. Evolutionary divergence and convergence in proteins, pp. 97–166 in Evolving Genes and Proteins. In Bryson, V. and Vogel, H.J. (eds.). Academic Press, New York, N.Y., USA.
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Verma, P., Seong, C.N., Pandey, P.K. et al. Bhargavaea indica sp. nov., a member of the phylum Firmicutes, isolated from Arabian Sea sediment. J Microbiol. 51, 36–42 (2013). https://doi.org/10.1007/s12275-013-2488-z