Journal of Microbiology

, Volume 51, Issue 1, pp 36–42

Bhargavaea indica sp. nov., a member of the phylum Firmicutes, isolated from Arabian Sea sediment

  • Pankaj Verma
  • Chi Nam Seong
  • Prashant Kumar Pandey
  • Ramesh Ramchandra Bhonde
  • Cathrin Spröer
  • Manfred Rohde
  • Yogesh Shreepad Shouche
Microbial Systematics and Evolutionary Microbiology

DOI: 10.1007/s12275-013-2488-z

Cite this article as:
Verma, P., Seong, C.N., Pandey, P.K. et al. J Microbiol. (2013) 51: 36. doi:10.1007/s12275-013-2488-z
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Abstract

A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, non-endospore-forming and grew with 0–8.5% (w/v) NaCl, at 15–48°C and at pH 6.5–9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98T forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA-DNA relatedness values of strain KJW98T with B. beijingensis DSM 19037T, B. cecembensis LMG 24411T and B. ginsengi DSM 19038T were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 (37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW98T should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98T (=KCTC 13583T =LMG 25219T).

Keywords

Bhargavaea indica Firmicutes 16S rRNA gene gyrB 

Supplementary material

12275_2013_2488_MOESM1_ESM.pdf (102 kb)
Supplementary material, approximately 101 KB.

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Pankaj Verma
    • 1
  • Chi Nam Seong
    • 2
  • Prashant Kumar Pandey
    • 1
  • Ramesh Ramchandra Bhonde
    • 3
  • Cathrin Spröer
    • 4
  • Manfred Rohde
    • 5
  • Yogesh Shreepad Shouche
    • 1
    • 6
  1. 1.Molecular Biology UnitNational Centre for Cell SciencePuneIndia
  2. 2.Department of Biology, College of Life Science and Natural ResourcesSunchon National UniversitySuncheonRepublic of Korea
  3. 3.Manipal Institute of Regenerative MedicineBangaloreIndia
  4. 4.Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHBraunschweigGermany
  5. 5.BraunschweigGermany
  6. 6.Microbial Culture CollectionNational Centre for Cell SciencePuneIndia
  7. 7.National Institute of Ocean TechnologyPallikaranai, ChennaiIndia

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