Skip to main content

Lysinibacillus tabacifolii sp. nov., a novel endophytic bacterium isolated from Nicotiana tabacum leaves

Journal of Microbiology volume 51, pages 289–294 (2013)Cite this article

Abstract

A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Ahmed, I., Yokota, A., Yamazoe, A., and Fujiwara, T. 2007. Proposal of Lysinibacillus boronitolerans gen. nov., sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.. Int. J. Syst. Evol. Microbiol.57, 1117–1125.

    PubMed  Article  CAS  Google Scholar 

  • Christensen, H., Angen, O., Mutters, R., Olsen, J.E., and Bisgaard, M. 2000. DNA-DNA hybridization determined in microwells using covalent attachment of DNA. Int. J. Syst. Evol. Microbiol.50, 1095–1102.

    PubMed  Article  CAS  Google Scholar 

  • Collins, M.D. and Jones, D. 1980. Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. J. Appl. Bacteriol.48, 459–470.

    Article  CAS  Google Scholar 

  • Collins, M.D., Pirouz, T., Goodfellow, M., and Minnikin, D.E. 1977. Distribution of menaquinones in actinomycetes and corynebacteria. J. Gen. Microbiol.100, 221–230.

    PubMed  Article  CAS  Google Scholar 

  • Ezaki, T., Hashimoto, Y., and Yabuuchi, E. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol.39, 224–229.

    Article  Google Scholar 

  • Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol.17, 368–376.

    PubMed  Article  CAS  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution39, 783–789.

    Article  Google Scholar 

  • Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool.20, 406–416.

    Article  Google Scholar 

  • Gregersen, T. 1978. Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur. J. Appl. Microbiol. Biotechnol.5, 123–127.

    Article  Google Scholar 

  • Groth, I., Schumann, P., Rainey, F.A., Martin, K., Schuetze, B., and Augsten, K. 1997. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int. J. Syst. Bacteriol.47, 1129–1133.

    PubMed  Article  CAS  Google Scholar 

  • Groth, I., Schumann, P., Weiss, N., Martin, K., and Rainey, F.A. 1996. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int. J. Syst. Bacteriol.46, 234–239.

    PubMed  Article  CAS  Google Scholar 

  • Guindon, S. and Gascuel, O. 2003. PhyML — A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol.52, 696–704.

    PubMed  Article  Google Scholar 

  • Jung, M.Y., Paek, W.K., Styrak, L., and Chang, Y.H. 2012. Proposal of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended descriptions of the genus. Int. J. Syst. Evol. Microbiol.62, 2347–2355.

    PubMed  Article  CAS  Google Scholar 

  • Katsivela, E., Bonse, D., Krüger, A., Strömpl, C., Livingston, A., and Ittich, R.M. 1999. An extractive membrane biofilm reactor fordegradation of 1,3-dichloropropene in industrial wastewater. Appl. Microbiol. Biotechnol.52, 853–862.

    PubMed  Article  CAS  Google Scholar 

  • Lee, C.S., Jung, Y.T., Park, S., Oh, T.K., and Yoon, J.H. 2010. Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus. Int. J. Syst. Evol. Microbiol.60, 281–286.

    PubMed  Article  CAS  Google Scholar 

  • Li, W.J., Xu, P., Schumann, P., Zhang, Y.Q., Pukall, R., Xu, L.H., Stackebrandt, E., and Jiang, C.L. 2007. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int. J. Syst. Evol. Microbiol.57, 1424–1428.

    PubMed  Article  Google Scholar 

  • Logan, N.A., Berge, O., Bishop, A.H., Busse, H.J., De Vos, P., Fritze, D., Heyndrickx, M., Kämpfer, P., Rabinovitch, L., Salkinoja-Salonen, M.S., andet al. 2009. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int. J. Syst. Evol. Microbiol.59, 2114–2121.

    PubMed  Article  CAS  Google Scholar 

  • MacFaddin, J.F. 1980. Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore, Williams & Wilkins, MD, USA.

    Google Scholar 

  • Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol.39, 159–167.

    Article  CAS  Google Scholar 

  • Miché, L. and Balandreau, J. 2001. Effects of rice seed surface sterilization withhypochlorite on inoculated Burkholderia vietnamiensis. Appl. Environ. Microbiol.67, 3046–3052.

    PubMed  Article  Google Scholar 

  • Minnikin, D.E., Collins, M.D., and Goodfellow, M. 1979. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J. Appl. Bacteriol.47, 87–95.

    Article  CAS  Google Scholar 

  • Miwa, H., Ahmed, I., Yokota, A., and Fujiwara, T. 2009. Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil. Int. J. Syst. Evol. Microbiol.59, 1427–1432.

    PubMed  Article  CAS  Google Scholar 

  • Qin, S., Li, J., Chen, H.H., Zhao, G.Z., Zhu, W.Y., Jiang, C.L., Xu, L.H., and Li, W.J. 2009. Isolation, diversity and antimicrobial activity of rare Actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl. Environ. Microbiol.75, 6176–6186.

    PubMed  Article  CAS  Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol. Biol. Evol.4, 406–425.

    PubMed  CAS  Google Scholar 

  • Schleifer, K.H. and Kandler, O. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev.36, 407–477.

    PubMed  CAS  Google Scholar 

  • Shirling, E.B. and Gottlieb, D. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol.16, 313–340.

    Article  Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., and Kumar. S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol. Biol. Evol.24, 1596–1599.

    PubMed  Article  CAS  Google Scholar 

  • Tang, S.K., Wang, Y., Chen, Y., Lou, K., Cao, L.L., Xu, L.H., and Li, W.J. 2009. Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int. J. Syst. Evol. Microbiol.59, 2025–2031.

    PubMed  Article  CAS  Google Scholar 

  • Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res.25, 4876–4882.

    PubMed  Article  CAS  Google Scholar 

  • Williams, S.T., Goodfellow, M., and Alderson, G. 1989. Genus Streptomyces Waksman and Henrici 1943, 339AL, Bergeys Manual of Systematic Bacteriology, vol. 4, pp. 2463–2468. In Williams, S.T., Sharpe, M.E., and Holt, J.G. (eds.) Williams & Wilkins, Baltimore, USA.

  • Yang, L.L., Huang, Y., Liu, J., Ma, L., Mo, M.H., Li, W.J., and Yang, F.X. 2012. Lysinibacillus mangiferihumi sp. nov., a new bacterium producing nematicidal volatiles. Antonie van Leeuwenhoek102, 53–59.

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Technology Center of Hongyun Honghe Tobacco (Group) Co., Ltd, Kunming, 650202, P. R. China

    Yan-Qing Duan, Song-Tao He, Ming-Feng Wang, Wen-Yuan Wang & Wei Zhe

  2. School of Life Sciences, Yunnan Normal University, Kunming, 650092, P. R. China

    Qing-Qing Li

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, P. R. China

    Yong-Hong Cao, Ming-He Mo & Wen-Jun Li

  4. Wudu BaYi High School, Gansu province, 746023, P. R. China

    Yu-Long Zhai

  5. Key Laboratory of Biogeography and Bio-resources in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, P. R. China

    Wen-Jun Li

Authors
  1. Yan-Qing Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Song-Tao He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qing-Qing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ming-Feng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wen-Yuan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei Zhe
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yong-Hong Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ming-He Mo
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yu-Long Zhai
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Wen-Jun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Wei Zhe or Wen-Jun Li.

Additional information

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

Electronic supplementary material

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Duan, YQ., He, ST., Li, QQ. et al. Lysinibacillus tabacifolii sp. nov., a novel endophytic bacterium isolated from Nicotiana tabacum leaves. J Microbiol. 51, 289–294 (2013). https://doi.org/10.1007/s12275-013-2338-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-013-2338-z

Keywords

  • Lysinibacillus tabacifolii
  • polyphasic taxonomy
  • Nicotiana tabacum