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Fosmid cloning, nucleotide sequence, and characterization of a beta-lactamase gene from subsurface isolates

Journal of Microbiology volume 50, pages 680–683 (2012)Cite this article

Abstract

A beta-lactamase gene was isolated for the first time from a terrestrial subsurface environment using a combined cultivation and direct cloning strategy. The gene, discovered from 24 m below land surface in Hawaii, was most similar to the penicillinase from Bacillus licheniformis. The resistance gene was confirmed via subcloning and its minimum inhibitory concentration values were measured against several test beta-lactam antibiotics. This study extends the knowledge on resistance to antimicrobials, which may help the efforts to minimize their future threat.

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References

  • Allen, H.K., Donato, J., Wang, H.H., Cloud-Hansen, K.A., Davies, J., and Handelsman, J. 2010. Call of the wild: antibiotic resistance genes in natural environments. Nature Rev. Microbiol. 8, 251–259.

    Article  CAS  Google Scholar 

  • Allen, H.K., Moe, L.A., Rodbumrer, J., and Gaarder, A. 2009. Functional metagenomics reveals diverse beta-lactamases in a remote Alaskan soil. ISME J. 3, 243–251.

    PubMed  Article  CAS  Google Scholar 

  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403–410.

    PubMed  CAS  Google Scholar 

  • Andrews, J.M. 2001. Determination of minumum inhibitory concentrations. J. Antimicrob. Chemother. 48, 5–16.

    PubMed  Article  CAS  Google Scholar 

  • Brown, M.G. and Balkwill, D.L. 2009. Antibiotic resistance in bacteria isolated from the deep terrestrial subsurface. Microb. Ecol. 57, 484–493.

    PubMed  Article  CAS  Google Scholar 

  • Canada, K.A., Iwashita, S., Shim, H., and Wood, T.K. 2002. Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. J. Bacteriol. 184, 344–349.

    PubMed  Article  CAS  Google Scholar 

  • Entcheva, P., Liebl, W., Johann, A., Hartsch, T., and Streit, W.R. 2001. Direct cloning from enrichment cultures, a reliable strategy for isolation of complete operons and genes from microbial consortia. Appl. Environ. Microbiol. 67, 89–99.

    PubMed  Article  CAS  Google Scholar 

  • Fonze, E., Vanhove, M., Dive, G., Sauvage, E., Frere, J.-M., and Charlier, P. 2002. Crystal structures of the Bacillus licheniformis BS3 class A b-lactamase and of the acyl-enzyme adduct formed with cefoxitin. Biochemistry 41, 1877–1885.

    PubMed  Article  CAS  Google Scholar 

  • Hawkey, P.M. and Jones, A.M. 2009. The changing epidemiology of resistance. J. Antimicrob. Chemother. 64, i3–i10.

    PubMed  Article  CAS  Google Scholar 

  • Maeda, T., Sanchez-Torres, V., and Wood, T.K. 2007. Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli. Appl. Microbiol. Biotechnol. 77, 879–890.

    PubMed  Article  CAS  Google Scholar 

  • Neugebauer, K., Sprengel, R., and Schaller, H. 1981. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium. Nucleic Acids Res. 9, 2577–2588.

    PubMed  Article  CAS  Google Scholar 

  • Parkes, R.J. and Wellsbury, P. 2004. Deep biospheres. In Bull, A.T. (ed.), Microbial Diversity and Bioprospecting, Vol. 12, pp. 120–129. ASM Press, Washington, D.C., USA.

    Google Scholar 

  • Phelps, T.J., Fliermans, C.B., Garland, T.R., Pfiffner, S.M., and White, D.C. 1989. Methods for recovery of deep terrestrial subsurface sediments for microbiological studies. J. Microb. Methods 9, 267–279.

    Article  Google Scholar 

  • Rey, M.W., Ramaiya, P., Nelson, B.A., Brody-Karpin, S.D., Zaretsky, E.J., Tang, M., Leon, A.L.d., Xiang, H., Gusti, V., Clausen, I.G., and et al. 2004. Complete genome sequence of the industrial bacterium Bacillus liceniformis and comparisons with closely related Bacillus species. Genome Biol. 5, R77.71–R77.12.

    Article  Google Scholar 

  • Vardar, N. and Vardar-Schara, G. 2011. A novel beta-lactamase from subsurface sediments. Curr. Opin. Biotechnol. 22, S123–S124.

    Article  Google Scholar 

  • Wright, G.D. 2010. Antibiotic resistance in the environment: a link to the clinic? Curr. Opin. Microbiol. 13, 589–594.

    PubMed  Article  CAS  Google Scholar 

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Affiliations

  1. Department of Genetics and Bioengineering, Fatih University, Büyükçekmece, Istanbul, 34500, Turkey

    Nurcan Vardar & Gölnül Vardar-Schara

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  1. Nurcan Vardar
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  2. Gölnül Vardar-Schara
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Correspondence to Gölnül Vardar-Schara.

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Vardar, N., Vardar-Schara, G. Fosmid cloning, nucleotide sequence, and characterization of a beta-lactamase gene from subsurface isolates. J Microbiol. 50, 680–683 (2012). https://doi.org/10.1007/s12275-012-2139-9

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  • DOI: https://doi.org/10.1007/s12275-012-2139-9

Keywords

  • beta-lactamase
  • terrestrial subsurface
  • antibiotic resistance
  • direct cloning