Skip to main content

Flavobacterium cheonhonense sp. nov., isolated from a freshwater reservoir

Abstract

A novel bacterium, designated strain ARSA-15T, was isolated from a freshwater sample collected from the Cheonho reservoir, Cheonan, Republic of Korea. The isolate was deep-yellow pigment, Gram-negative, rod-shaped, non-motile, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Flavobacterium, and shared less than 97% sequence similarity with recognized Flavobacterium species. The novel species was able to grow at 10–37°C, pH 6.5–10.0, and in 0–0.5% (w/v) NaCl concentrations. Chemotaxonomically, iso-C15:1, iso-C15:0, and iso-C16:0 were observed to be the predominant cellular fatty acid, and menaquinone-6 (MK-6) was the predominant respiratory quinone. The major polar lipid patterns of strain ARSA-19T was phosphatidylethanolamine, unknown aminolipid (AL1 and AL2), and unidentified polar lipids (L1, L2, and L3). The genomic DNA G+C content of the isolate was 39.2 mol%. On the basis of polyphasic approach, strain ARSA-15T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium cheonhonense sp. nov. is proposed. The type strain is ARSA-15T (=KACC 14967T =KCTC 23180T =JCM 17064T).

This is a preview of subscription content, access via your institution.

References

  • Anacker, R.L. and Ordal, E.J. 1955. Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J. Bacteriol.70, 738–741.

    PubMed  CAS  Google Scholar 

  • Bandelt, H.J., Forster, P., and Rohl, A. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol.16, 37–48.

    PubMed  Article  CAS  Google Scholar 

  • Bergey, D.H., Harrison, F.C., Breed, R.S., Hammer, B.W., and Huntoon, F.M. 1923. Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore, USA.

    Google Scholar 

  • Bernardet, J.F. and Bowman, J.P. 2011. Genus I. Flavobacterium Bergey et al. 1923. In Whitman, W. (ed.), Bergey’s Manual of Systematic Bacteriology, pp. 112–154, 2nd ed., Vol. 4, The Williams & Wilkins Co., Baltimore, USA.

    Google Scholar 

  • Bernardet, J.F., Nakagawa, Y., and Holmes, B. 2002. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol.52, 1049–1070.

    PubMed  Article  CAS  Google Scholar 

  • Bernardet, J.F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., and Vandamme, P. 1996. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the Family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Evol. Microbiol. 46, 128–148.

  • Chun, J. and Bae, K.S. 2000. Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences. Antonie van Leeuwenhoek78, 123–127.

    PubMed  Article  CAS  Google Scholar 

  • Chun, J.S., Lee, J.H., Jung, Y.Y., Kim, M., Kim, S., Kim, B.K., and Lim, Y.W. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol.57, 2259–2261.

    PubMed  Article  CAS  Google Scholar 

  • Cousin, S., Puker, O., and Stackebrandt, E. 2007. Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int. J. Syst. Evol. Microbiol.57, 243–249.

    PubMed  Article  CAS  Google Scholar 

  • Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser.41, 95–98.

    CAS  Google Scholar 

  • Kim, J.H., Kim, K.Y., and Cha, C.J. 2009. Flavobacterium chungangense sp. nov., isolated from a freshwater lake. Int. J. Syst. Evol. Microbiol.59, 1754–1758.

    PubMed  Article  CAS  Google Scholar 

  • Lane, D.J. 1991. 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics, pp. 115–175. In Stackebrandt, E. and Goodfellow, M. (eds.). Wiley, Chichester, UK.

    Google Scholar 

  • Lee, S., Weon, H.Y., Kim, S.J., and Ahn, T.Y. 2011. Flavobacterium koreense sp. nov., Flavobacterium chungnamense sp. nov. and Flavobacterium cheonanense sp. nov., isolated from a freshwater reservoir. J. Microbiol.49, 387–392.

    PubMed  Article  Google Scholar 

  • Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol.39, 159–167.

    Article  CAS  Google Scholar 

  • Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods2, 233–241.

    Article  CAS  Google Scholar 

  • Moore, D.D. and Dowhan, D. 1995. Preparation and analysis of DNA. Current Protocols in Molecular Biology, pp. 2–11. In Ausubel, F.W., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.). Wiley, New York, N.Y., USA.

    Google Scholar 

  • Perry, L.B. 1973. Gliding motility in some non-spreading Flexibacteria. J. Appl. Bacteriol.36, 227–232.

    PubMed  Article  CAS  Google Scholar 

  • Qu, J.H., Yuan, H.L., Li, H.F., and Deng, C.P. 2009. Flavobacterium cauense sp. nov., isolated from sediment of a eutrophic lake. Int. J. Syst. Evol. Microbiol.59, 2666–2669.

    PubMed  Article  CAS  Google Scholar 

  • Skerman, V.B.D. 1967. A guide to the identification of the Genera of Bacteria, 2nd edn. Williams & Wilkins, Baltimore, USA.

    Google Scholar 

  • Smibert, R.M. and Krieg, N.R. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology, pp. 607–654. In Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. (eds.). American Society for Microbiology, Washington, D.C., USA.

    Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol.24, 1596–1599.

    PubMed  Article  CAS  Google Scholar 

  • Wang, Z.W., Liu, Y.H., Dai, X., Wang, B.J., Jiang, C.Y., and Liu, S.J. 2006. Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int. J. Syst. Evol. Microbiol.56, 439–442.

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tae-Young Ahn.

Additional information

Supplemental material for this article may be found at http://www.springer.com/content/120956

Electronic supplementary material

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, S., Oh, JH., Weon, HY. et al. Flavobacterium cheonhonense sp. nov., isolated from a freshwater reservoir. J Microbiol. 50, 562–566 (2012). https://doi.org/10.1007/s12275-012-1229-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-012-1229-z

Keywords

  • freshwater
  • phylogenetic analysis
  • Flavobacterium