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Immunostimulatory Activity of Dendritic cells pulsed with carbonic anhydrase IX and Acinetobacter baumannii outer membrane protein A

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Abstract

Dendritic cell (DC)-based immunotherapy is a potent therapeutic modality for treating renal cell carcinoma (RCC), but development of antigens specific for tumor-targeting and anti-tumor immunity is of great interest for clinical trials. The present study investigated the ability of DCs pulsed with a combination of carbonic anhydrase IX (CA9) as an RCC-specific biomarker and Acinetobacter baumannii outer membrane protein A (AbOmpA) as an immunoadjuvant to induce anti-tumor immunity against murine renal cell carcinoma (RENCA) in a murine model. Murine bone-marrow-derived DCs pulsed with a combination of RENCA lysates and AbOmpA were tested for their capacity to induce DC maturation and T cell responses in vitro. A combination of RENCA lysates and AbOmpA up-regulated the surface expression of co-stimulatory molecules, CD80 and CD86, and the antigen presenting molecules, major histocompatibility (MHC) class I and class II, in DCs. A combination of RENCA lysates and AbOmpA also induced interleukin-12 (IL-12) production in DCs. Next, the immunostimulatory activity of DCs pulsed with a combination of CA9 and AbOmpA was determined. A combination of CA9 and AbOmpA up-regulated the surface expression of co-stimulatory molecules and antigen presenting molecules in DCs. DCs pulsed with a combination of CA9 and AbOmpA effectively secreted IL-12 but not IL-10. These cells interacted with T cells and formed clusters. DCs pulsed with CA9 and AbOmpA elicited the secretion of interferon-γ and IL-2 in T cells. In conclusion, a combination of CA9 and AbOmpA enhanced the immunostimulatory activity of DCs, which may effectively induce anti-tumor immunity against human RCC.

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Authors and Affiliations

  1. Department of Physiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Bo Ra Kim, Eun Kyoung Yang & Sun Hee Kim

  2. Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Bo Ra Kim, Dong Chan Moon & Je Chul Lee

  3. Department of Microbiology, Chungnam National University, College of Medicine, Daejeon, 301-747, Republic of Korea

    Hwa Jung Kim

  4. Department of Urology, Catholic University of Daegu, School of Medicine, Daegu, 705-718, Republic of Korea

    Duk Yoon Kim

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  1. Bo Ra Kim
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  2. Eun Kyoung Yang
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  4. Dong Chan Moon
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Correspondence to Je Chul Lee or Duk Yoon Kim.

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These authors contributed equally to this work.

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Kim, B.R., Yang, E.K., Kim, S.H. et al. Immunostimulatory Activity of Dendritic cells pulsed with carbonic anhydrase IX and Acinetobacter baumannii outer membrane protein A. J Microbiol. 49, 115–120 (2011). https://doi.org/10.1007/s12275-011-1037-x

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  • DOI: https://doi.org/10.1007/s12275-011-1037-x

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