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A modified immunoblot method to identify substrates of protein kinases

The Journal of Microbiology volume 49, pages 499–501 (2011)Cite this article

Abstract

While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.

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Authors and Affiliations

  1. Department of Biological Science, Wayne State University, 5047 Gullen Mall, Detroit, MI, 48202, USA

    Choong-Min Kang

  2. Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA

    Wan Jin Jahng

  3. Division of Infectious Diseases, Children’s Hospital Boston, Harvard Medical School, 300 Longwood Ave., Boston, MA, 02115, USA

    Robert N. Husson

  4. Department of Biological Sciences, Myongji University, Gyeonggido, 449-728, Republic of Korea

    Sang Hee Lee

Authors
  1. Choong-Min Kang
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  2. Wan Jin Jahng
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  3. Robert N. Husson
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  4. Sang Hee Lee
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Corresponding authors

Correspondence to Choong-Min Kang or Sang Hee Lee.

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Kang, CM., Jahng, W.J., Husson, R.N. et al. A modified immunoblot method to identify substrates of protein kinases. J Microbiol. 49, 499–501 (2011). https://doi.org/10.1007/s12275-011-0465-y

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  • DOI: https://doi.org/10.1007/s12275-011-0465-y

Keywords

  • modified immunoblot method
  • substrates of kinases
  • partial transfer of proteins

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