Oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of Xinjiang, China

Abstract

Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively. The strains grew optimally at 30–37°C, pH 8–11, in the presence of 5–10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1–99.2% similarity to O. kapialis KCTC 13177T, 99.2–99.3% to O. picturae KCTC 3821T, and 94.2–96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other, but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine and hydroxy-phosphatidyl ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7–40.1 mol%. On the basis of these results, the three strains should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp. nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T).

This is a preview of subscription content, access via your institution.

References

  1. Chen, W., E. Wang, S. Wang, Y. Li, X. Chen, and Y. Li. 1995. Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People’s Republic of China. Int. J. Syst. Bacteriol. 45, 153–159.

    Article  CAS  PubMed  Google Scholar 

  2. Collins, M.D. 1985. Isoprenoid quinone analysis in classification and identification. Chemical Methods in Bacterial Systematics, pp. 267–287. In M. Goodfellow and D.E. Minnikin (eds.). Academic Press, London, UK.

    Google Scholar 

  3. De Ley, J., H. Cattoir, and A. Reynaerts. 1970. The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12, 133–142.

    Article  PubMed  Google Scholar 

  4. Dong, X.Z. and M.Y. Cai. 2001. Determination of biochemical properties. Manual for Systematic Identification of General Bacteria, pp. 370–398. Science Press, Beijing, China.

    Google Scholar 

  5. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.

    Article  Google Scholar 

  6. Heyrman, J., N.A. Logan, H.J. Busse, A. Balcaen, L. Lebbe, M. Rodriguez-Diaz, J. Swings, and P. De Vos. 2003. Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int. J. Syst. Bacteriol. 53, 501–511.

    CAS  Google Scholar 

  7. Kim, Y.G., D.H. Choi, S. Hyun, and B.C. Cho. 2007. Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int. J. Syst. Evol. Microbiol. 57, 409–413.

    Article  CAS  PubMed  Google Scholar 

  8. Lee, J.S., J.M. Lim, K.C. Lee, J.C. Lee, Y.H. Park, and C.J. Kim. 2006. Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int. J. Syst. Evol. Microbiol. 56, 251–257.

    Article  CAS  PubMed  Google Scholar 

  9. Lee, S.Y., T.K. Oh, W. Kim, and J.H. Yoon. 2010. Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern of the Yellow Sea, Korea. Int. J. Syst. Evol. Microbiol. Article in press. 2010 as doi:10.1099/ijs.0.021907-0.

  10. Liu, W.Y., J. Zeng, L. Wang, Y.T. Dou, and S.S. Yang. 2005. Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int. J. Syst. Evol. Microbiol. 55, 1991–1996.

    Article  CAS  PubMed  Google Scholar 

  11. Logan, N.A., O. Berge, A.H. Bishop, H.J. Busse, P. De Vos, D. Fritze, M. Heyndrickx, and et al. 2009. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int. J. Syst. Evol. Microbiol. 59, 2114–2121.

    Article  CAS  PubMed  Google Scholar 

  12. Lu, J., Y. Nogi, and H. Takami. 2001. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol. Lett. 205, 291–297.

    Article  CAS  PubMed  Google Scholar 

  13. Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208–218.

    Article  CAS  Google Scholar 

  14. Marmur, J. and P. Doty. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5, 109–118.

    Article  CAS  PubMed  Google Scholar 

  15. Munro, H.N. 1969. Evolution of protein molecules. Mammalian Protein Metabolism, pp. 21–132. In H.N. Munro (ed.). Academic Press, New York, N.Y., USA.

    Google Scholar 

  16. Nam, J.H., W. Bae, and D.H. Lee. 2008. Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int. J. Syst. Evol. Microbiol. 58, 1109–1113.

    Article  CAS  PubMed  Google Scholar 

  17. Namwong, S., S. Tanasupawat, K.C. Lee, and J.S. Lee. 2009. Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int. J. Syst. Evol. Microbiol. 59, 2254–2259.

    Article  CAS  PubMed  Google Scholar 

  18. Raats, D. and M. Halpern. 2007. Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int. J. Syst. Evol. Microbiol. 57, 255–259.

    Article  CAS  PubMed  Google Scholar 

  19. Romano, I., L. Lama, B. Nicolaus, A. Poli, A. Gambacorta, and A. Giordano. 2006. Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. Int. J. Syst. Evol. Microbiol. 56, 805–810.

    Article  CAS  PubMed  Google Scholar 

  20. Saitou, N. and M. NeiM. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.

    CAS  PubMed  Google Scholar 

  21. Schleifer, K.H. 1985. Analysis of the chemical composition and primary structure of murein. Methods Microbiol. 18, 123–156.

    Article  CAS  Google Scholar 

  22. Schleifer, K.H. and O. Kandler. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36, 407–477.

    CAS  PubMed  Google Scholar 

  23. Spring, S., W. Ludwig, M.C. Marquez, A. Ventosa, and K.H. Schleifer. 1996. Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int. J. Syst. Bacteriol. 46, 492–496.

    Article  Google Scholar 

  24. Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.

    Article  CAS  PubMed  Google Scholar 

  25. Tan, Z.Y., X.D. Xu, E.T. Wang, J.L. Gao, E. Martinez-Romero, and W.X. Chen. 1997. Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int. J. Syst. Bacteriol. 47, 874–879.

    Article  CAS  PubMed  Google Scholar 

  26. Tominaga, T., S.Y. An, H. Oyaizu, and A. Yokota. 2009. Oceanobacillus soja sp. nov. isolated from sauce production equipment in Japan. J. Gen. Appl. Microbiol. 55, 225–232.

    Article  CAS  PubMed  Google Scholar 

  27. Vauterin, L. and P. Vauterin. 1992. Computer-aided objective comparison of electrophoresis patterns for grouping and identifycation of microorganisms. Eur. Microbiol. 1, 37–41.

    Google Scholar 

  28. Ventosa, A., M.C. Marquez, M. Kocur, and B.J. Tindall. 1993. Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus. Int. J. Syst. Bacteriol. 43, 245–248.

    Article  CAS  PubMed  Google Scholar 

  29. Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.I. Krichevsky, L.H. Moore, and et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.

    Article  Google Scholar 

  30. Yang, J.Y., Y.Y. Huo, X.W. Xu, F.X. Meng, M. Wu, and C.S. Wang. 2009. Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. Int. J. Syst. Evol. Microbiol. Article in press.0: ijs.0.016295-0v1-ijs.0.016295-0.

  31. Yumoto, I., K. Hirota, Y. Nodasaka, and K. Nakajima. 2005. Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus. Int. J. Syst. Evol. Microbiol. 55, 1521–1524.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Su-Sheng Yang.

Additional information

These authors contributed equally to this work.

Supplemental material for this article may be found at http://www,springer.com/content/120956

Electronic supplementary material

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wang, L., Liu, WY., Gu, ZJ. et al. Oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of Xinjiang, China. J Microbiol. 48, 312–317 (2010). https://doi.org/10.1007/s12275-010-0135-5

Download citation

Keywords

  • Oceanobacillus manasiensis sp. nov.
  • moderate halophile
  • salt lake
  • Gram-positive