A strictly aerobic, red-pigmented, non-motile, catalase- and oxidase-positive, Gram-staining-negative bacterium, designated strain CNURIC011T, was isolated from seawater off the coast of Jeju Island in Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNURIC011T belongs to the genus Aquimarina in the family Flavobacteriaceae. 16S rRNA gene sequence analysis revealed that the close relatives of the novel strain are Aquimarina latercula ATCC 23177T, Aquimarina marcrocephali JAMB N27T, Aquimarina intermedia KMM 6258T, Aquimarina muelleri KMM 6020T, and Aquimarina brevivitae SMK-19T, with sequence similarities of 97.6, 96.6, 96.0, 95.6, and 94.2%, respectively. DNA-DNA hybridization revealed that the level of relatedness between strain CNURIC011T and Aquimarina latercula ATCC 23177T (=KCTC 2912T) was 4.9%. The DNA G+C content was 35.8 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C15:0 (14.9%), C15:0 (13.9%), iso-C17:0 3-OH (12.6%), iso-C15:1 G (7.3%), and iso-C17:1 ω9c (7.2%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC011T represents a novel species within the genus Aquimarina, for which the name Aquimarina litoralis sp. nov. is proposed. The type strain is CNURIC011T (=KCTC 22614T =JCM 15974T).
This is a preview of subscription content,to check access.
Access this article
Bernardet, J.F., Y. Nakagawa, and B. Holmes. 2002. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int. J. Syst. Evol. Microbiol. 52, 1049–1070.
Brown, T. 2005. Dot and slot blotting of DNA onto a positively charged nylon membrane using a manifold. Current Protocols in Molecular Biology, pp. 2.9.18–2.9.20. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl (eds.). Wiley, New York, N.Y., USA.
Chun, S.J., J.H. Lee, Y.Y. Jung, M.J. Kim, S.I. Kim, B.K. Kim, and Y.W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261.
Cole, J.R., B. Chai, T.L. Marsh, R.J. Farris, Q. Wang, S.A. Kulam, S. Chandra, D.M. McGarrell, T.M. Schmidt, G.M. Garrity, and J.M. Tiedje. 2003. The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31, 442–443.
Felsenstein, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.
Jeon, C.O., W. Park, W.C. Ghiorse, and E.L. Madsen. 2004. Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int. J. Syst. Evol. Microbiol. 54, 93–97.
Kimura, M. 1983. The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
Komagata, K. and K. Suzuki. 1987. Lipid and cell-wall analysis in bacterial systematic. Methods Microbiol. 19, 161–207.
Lane, D.J. 1991. 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. In E. Stackebrandt and M. Goodfellow (eds.). Wiley, New York, N.Y., USA.
Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19, 1–67.
Lewin, R.A. 1969. A classification of flexibacteria. J. Gen. Microbiol. 58, 189–206.
Marmur, J. and P. Doty. 1962. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5, 109–118.
McCammon, S.A. and J.P. Bowman. 2000. Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 50, 1055–1063.
Miyazaki, M., Y. Nagano, Y. Fujiwara, Y. Hatada, and Y. Nogi. 2009. Aquimarina marcrocephali sp. nov., isolated from the sediment agjacent to sperm whale carcasses off Kagoshima, Japan. Int. J. Syst. Evol. Microbiol. Article in press.
Nedashkovskaya, O.I., S.B. Kim, A.M. Lysenko, G.M. Frolova, V.V. Mikhailov, K.H. Lee, and K.S. Bae. 2005. Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 55, 225–229.
Nedashkovskaya, O.I., M. Vancanneyt, L. Christiaens, N.I. Kalinovskaya, V.V. Mikhailov, and J. Swings. 2006. Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina. Int. J. Syst. Evol. Microbiol. 56, 2037–2041.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876–4882.
Tindall, B.J., J. Sikorski, R.A. Simbert, and N.R. Kreig. 2007. Phenotypic characterization and principles of comparative systematics. Methods for General and Molecular Microbiology, pp. 330–393. In C.A. Reddy, T.J. Beveridge, J.A. Breznak, G.A. Marzluf, T.M. Schmidt, and L.R. Snyder (eds.). American Society for Microbiology, Washington, D.C., USA.
Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.I. Krichevsky, L.H. Moore, and et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.
Yoon, J.H., S.J. Kang, S.Y. Jung, H.W. Oh, and T.K. Oh. 2006. Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. Int. J. Syst. Evol. Microbiol. 56, 115–119.
ZoBell, C.E. 1941. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4, 42–75.
About this article
Cite this article
Oh, YS., Kahng, HY., Lee, Y.S. et al. Aquimarina litoralis sp. nov., isolated from a coastal seawater. J Microbiol. 48, 433–437 (2010). https://doi.org/10.1007/s12275-010-0088-8