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Characterization and identification of distinct Mycobacterium massiliense extracellular proteins from those of Mycobacterium abscessus

The Journal of Microbiology volume 48, pages 502–511 (2010)Cite this article

Abstract

Mycobacterium massiliense is an emerging pathogen and very similar to Mycobacterium abscessus of rapidly growing mycobacteria in the phenotype and genotype. Pathogenic bacteria secrete a diversity of factors into extracellular medium which contribute to the bacterial pathogenicity. In the present study, we performed the comparative proteome analysis of culture filtrate proteins from a clinical isolate of M. massiliense and M. abscessus strains using two-dimensional gel electrophoresis and liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). Interestingly, 9 proteins of M. massiliense were distinctly expressed from those of M. abscessus. Bioinformatic analysis of the identified proteins revealed that 3 unique proteins corresponded to serine/arginine rich protein, membrane protein from Streptomyces coelicolor, and one hypothetical protein from Corynebacterium efficiens YS-314, respectively. Culture filtrate proteins from M. massiliense induced the release of pro-inflammatory cytokines from macrophages in a dose-dependent manner but not that from M. abscessus. Taken together, the functional study on the identified proteins uniquely produced from M. massiliense may provide not only the clues for the different pathogensis, but also help develop the diagnostic tools for the differentiation between two mycobacterial species.

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Affiliations

  1. Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, 301-747, Republic of Korea

    A-Rum Shin, Hosung Sohn, Choul Jae Won, Byungsoo Lee, Woo Sik Kim, Hyun Bae Kang, Hwa-Jung Kim & Sung Jae Shin

  2. Department of Microbiology and Institute of Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, 120-749, Republic of Korea

    Sang Nae Cho

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  1. A-Rum Shin
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  2. Hosung Sohn
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  3. Choul Jae Won
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  4. Byungsoo Lee
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  5. Woo Sik Kim
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  6. Hyun Bae Kang
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  7. Hwa-Jung Kim
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  8. Sang Nae Cho
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  9. Sung Jae Shin
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Correspondence to Sung Jae Shin.

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Shin, AR., Sohn, H., Won, C.J. et al. Characterization and identification of distinct Mycobacterium massiliense extracellular proteins from those of Mycobacterium abscessus . J Microbiol. 48, 502–511 (2010). https://doi.org/10.1007/s12275-010-0038-5

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  • DOI: https://doi.org/10.1007/s12275-010-0038-5

Keywords

  • M. massiliense
  • extracellular proteins