Abstract
A pink-pigmented bacterium, designated SW08-7T was isolated from the drinking water of a water purifier. Cells were Gram-negative, rod-shaped, strictly aerobic, and non-spore-forming. It grew optimally at 25°C, pH 6∼7. Phylogenese analysis based on 16S rRNA gene sequence showed that strain SW08-7T belongs to the genus Methylobacterium. The highest 16S rRNA gene sequence similarities were found to Methylobacterium mesophilicum JCM 2829T (96.9%), Methylobacterium brachiatum B0021T (96.9%), Methylobacterium phyllosphaerae CBMB27T (96.6%), Methylobacterium radiotolerans JCM 2831T (96.6%), and Methylobacterium hispanicum GP34T (96.5%). DNA-DNA hybridization experiment revealed low-level (28.5%) of DNA-DNA relatedness between strain SW08-7T and Methylobacterium hispanicum. The genomic DNA G+C content was 68.9 mol% and the major isoprenoid quinone was Q-10. The major cellular fatty acid of strain SW08-7T was C18:1 ω7c (79.8±2.1%). Results of phylogenetic, phenotypic, and biochemical analyses revealed that strain SW08-7T could be classified as representing a novel species of genus Methylobacterium, for which the name Methylobacterium dankookense sp. nov. is proposed. The type strain is SW08-7T (=KCTC 22512T =DSM 224151).
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References
Baik, K.S., Y.D. Park, C.N. Sung, E.M. Kim, K.S. Bae, and J.S. Chun. 2006. Glaciecola nitratireducens sp. nov. isolated from seawater. Int. J. Syst. Evol. Microbiol. 56, 2185–2188.
Gallego, V., M.T. García, and A. Ventoa. 2005a. Methylobacterium hispanicum sp. nov. and methylobacterium aquaticum sp. nov., isolated from drinking water. Int. J. Syst. Evol. Microbiol. 55, 281–287.
Gallego, V., M.T. García, and A. Ventosa. 2005b. Methylobacterium variabile sp. nov., a methylotropic bacterium isolated from an aquatic environment. Int. J. Syst. Evol. Microbiol. 55, 1429–1433.
Gallego, V., M.T. García, and A. Ventosa. 2006. Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water. Int. J. Syst. Evol. Microbiol. 56, 339–342.
Green, P.N. 1992. The genus Methylobacterium, The Prokaryotes, 2nd ed., p. 2342–2349. In A. Balows, H.G. Truper, M. Dworkin, W. Harderand, and K.H. Schleifer (eds.). Springer, New York, N.Y., USA.
Green, P.N. and I.J. Bousfield. 1983. Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov., corrig.; Methylobacterium radiotolerans (Ito and lizuka 1971) comb. nov., corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov. Int. J. Syst. Bacteriol. 33, 875–877.
Hirashi, A., Y. Ueda, J. Ishihara, and T. Mori. 1996. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J. Gen. Appl. Microbiol. 42, 457–469.
Jourand, P., E. Giraud, G. Béna, A. Sy, A. Willems, M. Gillis, B. Dreyfus, and D.P. Lajudie. 2004. Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively Methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int. J. Syst. Evol. Microbiol. 54, 2269–2273.
Kato, Y., M. Asahara, K. Goto, H. Kasai, and A. Yokota. 2008. Methylobacterium persicinum sp. nov., Methylobacterium komagatae sp. nov., Methylobacterium brachiatum sp. nov., Methylobacterium tardum sp. nov. and Methylobacterium gregans sp. nov., isolated from freshwater. Int. J. Syst. Evol. Microbiol. 58, 1134–1141.
Madhaiyan, M., S. Poonguzhali, S.W. Kwon, and T.M. Sa. 2009. Methylobacterium phyllosphaerae sp. nov., a pinkpigmented, facultative methylotroph from the phyllosphere of rice. Int. J. Syst. Evol. Microbiol. 59, 22–27.
Mesbah, M., U. Premachandran, and W.B. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.
Moore, D.D. and D. Dowhan. 1995. Preparation and analysis of DNA, Current Protocols in Molecular Biology, p. 2–11. In F.W. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl (eds.). Wiley, New York, N.Y., USA.
Patt, T.E., G.C. Coole, and R.S. Hanson. 1976. Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int. J. Syst. Bacteriol. 26, 226–229.
Seldin, L. and D. Dubnau. 1985. Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int. J. Syst. Bacteriol. 35, 151–154.
Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.
Van Aken, B., C.M. Peres, S. Lafferty-Doty, J.M. Yoon, and J.L. Schnoor. 2004. Methylobacterium populi sp. nov., a novelaerobic, pink-pigmented, facultatively methylotrophic, ethaneutilizing bacterium isolated from poplar trees (Populus deltodes6nigra DN34). Int. J. Syst. Evol. Microbiol. 54, 1191–1196.
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Lee, SW., Oh, HW., Lee, KH. et al. Methylobacterium dankookense sp. nov., isolated from drinking water. J Microbiol. 47, 716–720 (2009). https://doi.org/10.1007/s12275-009-0126-6
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DOI: https://doi.org/10.1007/s12275-009-0126-6