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Iso-superoxide dismutase in Deinococcus grandis, a UV resistant bacterium

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Abstract

Deinococcus grandis possesses two types of superoxide dismutase (SOD, E. C. 1.15.1.1.) that show distinct electrophoretic behavior, one that migrates slowly and the other that migrates rapidly (SOD-1 and SOD-2, respectively). In this study, SOD-1 was uniformly and abundantly detected, regardless of growth phase, whereas SOD-2 was not detected during early growth, but was detectable from the exponential growth phase. In addition, a substantial increase in SOD-2 was observed in cells that were treated with potassium superoxide or UV, which suggests that SOD-2 is an inducible protein produced in response to stressful environments. Insensitivity of SOD-1 to both H2O2 and cyanide treatment suggests that SOD-1 is MnSOD. However, SOD-2 would be FeSOD, since it lost activity in response to H2O2 treatment, but not to cyanide. Localization studies of D. grandis iso-SODs in sucrose-shocked cells suggest that SOD-1 is a membrane-associated enzyme, whereas SOD-2 is a cytosolic enzyme. In conclusion, SOD-1 seems to be an essential constitutive enzyme for viability and SOD-2 appears to be an inducible enzyme that is probably critical for survival upon UV irradiation and oxidative stress.

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  1. Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Na-Rae Yun & Young Nam Lee

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  1. Na-Rae Yun
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Yun, NR., Lee, Y.N. Iso-superoxide dismutase in Deinococcus grandis, a UV resistant bacterium. J Microbiol. 47, 172–177 (2009). https://doi.org/10.1007/s12275-008-0221-0

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