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Induction of IL-8 in periodontal ligament cells by H2O2

The Journal of Microbiology volume 46, pages 579–584 (2008)Cite this article

Abstract

Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H2O2, one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H2O2. IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH2-terminal kinase (JNK) was estimated by Western blotting. Treatment with H2O2 at concentration of up to 250 μM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 μM H2O2 did not increase IL-8 production. Catalase, an inhibitor of H2O2, down-regulated the production of IL-8 induced by H2O2. H2O2 increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H2O2. These results indicate that H2O2 acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H2O2 deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.

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Authors and Affiliations

  1. Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry, Seoul, 120-752, Republic of Korea

    Yang-Sin Lee, Eun Jung Bak, Minyoung Kim, Jeong Taeg Seo & Yun-Jung Yoo

  2. Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, 120-752, Republic of Korea

    Minyoung Kim, Jeong Taeg Seo & Yun-Jung Yoo

  3. Department of General Dentistry, Dental Hospital, Yonsei University, Seoul, 120-752, Republic of Korea

    Wonse Park

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  1. Yang-Sin Lee
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  2. Eun Jung Bak
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  3. Minyoung Kim
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  4. Wonse Park
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  5. Jeong Taeg Seo
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Correspondence to Yun-Jung Yoo.

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Lee, YS., Bak, E.J., Kim, M. et al. Induction of IL-8 in periodontal ligament cells by H2O2 . J Microbiol. 46, 579–584 (2008). https://doi.org/10.1007/s12275-008-0182-3

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  • DOI: https://doi.org/10.1007/s12275-008-0182-3

Keywords

  • IL-8
  • periodontal ligament cells
  • H2O2