The in vivo targeted molecular imaging of fluorescent silicon nanoparticles in Caenorhabditis elegans
- 190 Downloads
Owing to their unique optical properties (e.g., bright fluorescence coupled with strong photostability) and negligible toxicity, fluorescent silicon nanoparticles (SiNPs) have been demonstrated to be promising probes for bioimaging analysis. Herein, we describe the use of Caenorhabditis elegans (C. elegans) as an animal model to investigate the in vivo behavior and molecular imaging capacity of ultrasmall fluorescent SiNPs (e.g., ∼3.9 ± 0.4 nm). Our studies show that (1) the internalized SiNPs do not affect the morphology and physiology of the worms, suggesting the superior biocompatibility of SiNPs in live organisms; (2) the internalized SiNPs cannot cross the basement membrane of C. elegans tissues and they display limited diffusion ability in vivo, providing the possibility of their use as nanoprobes for specific tissue imaging studies in intact animals; (3) more than 80% of the fluorescence signal of internalized SiNPs remains even after 120 min of continuous laser bleaching, whereas only ∼20% of the signal intensity of mCherry or cadmium telluride quantum dots remains under the same condition, indicating the robust photostability of SiNPs in live organisms; and (4) cyclic RGD-peptide-conjugated SiNPs can specifically label muscle attachment structures in live C. elegans, which is the first proof-of-concept example of SiNPs for targeted molecular imaging in these live worms. These finding raise exciting opportunities for the design of high-quality SiNP-based fluorescent probes for long-term and real-time tracking of biological events in vivo.
Keywordsfluorescent silicon nanoparticles molecular imaging Caenorhabditis elegans in vivo behavior
Unable to display preview. Download preview PDF.
The authors would like to thank X. Wang and the CGC for reagents, Y. Zhu, Y. Li, W. Li and L. Li (Soochow University, China) for technical assistance. This work was supported by grants from National Basic Research Program of China (Nos. 2013CB934400 and 2012CB932400), the National Natural Science Foundation of China (Nos. 61361160412, 21575096, 31271429, 21605109 and 31400860), the Natural Science Foundation of Jiangsu Province of China (Nos. BK20130052, BK20130298 and BK20160009), Jiangsu Provincial Innovative Research Team and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1075), 111 Project and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), as well as the Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC).
- Xiao, L. S.; Li, J. T.; Brougham, D. F.; Fox, E. K.; Feliu, N.; Bushmelev, A.; Schmidt, A.; Mertens, N.; Kiessling, F.; Valldor, M. et al. Water-soluble superparamagnetic magnetite nanoparticles with biocompatible coating for enhanced magnetic resonance imaging. ACS Nano 2011, 5, 6315–6324.CrossRefGoogle Scholar
- Eberlin, L. S.; Tibshirani, R. J.; Zhang, J. L.; Longacre, T. A.; Berry, G. J.; Bingham, D. B.; Norton, J. A.; Zare, R. N.; Poultsides, G. A. Molecular assessment of surgical-resection margins of gastric cancer by mass-spectrometric imaging. Proc. Natl. Acad. Sci. USA 2014, 111, 2436–2441.CrossRefGoogle Scholar
- Dasog, M.; Yang, Z. Y.; Regli, S.; Atkins, T. M.; Faramus, A.; Singh, M. P.; Muthuswamy, E.; Kauzlarich, S. M.; Tilley, R. D.; Veinot, J. G. C. Chemical insight into the origin of red and blue photoluminescence arising from freestanding silicon nanocrystals. ACS Nano 2013, 7, 2676–2685.CrossRefGoogle Scholar
- Zhang, X. D.; Luo, Z. T.; Chen, J.; Song, S. S.; Yuan, X.; Shen, X.; Wang, H.; Sun, Y. M.; Gao, K.; Zhang, L. F. et al. Ultrasmall glutathione-protected gold nanoclusters as next generation radiotherapy sensitizers with high tumor uptake and high renal clearance. Sci. Rep. 2015, 5, 8669.CrossRefGoogle Scholar
- Liu, J.; Wang, P. Y.; Zhang, X.; Wang, L. M.; Wang, D. L.; Gu, Z. J.; Tang, J. L.; Guo, M. Y.; Cao, M. J.; Zhou, H. G. et al. Rapid degradation and high renal clearance of Cu3BiS3 nanodots for efficient cancer diagnosis and photothermal therapy in vivo. ACS Nano 2016, 10, 4587–4598.CrossRefGoogle Scholar