Folate targeted coated SPIONs as efficient tool for MRI
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The development of more sensitive diagnostic tools allowing an early-stage and highly efficient medical imaging of tumors remains a challenge. Magnetic nanoparticles seem to be the contrast agents with the highest potential, if properly constructed. Therefore, in this study, hybrid magnetic nanoarchitectures were developed using a new amphiphilic inulin-based graft copolymer (INU-LAPEG-FA) as coating material for 10-nm spinel iron oxide (magnetite, Fe3O4) superparamagnetic nanoparticles (SPION). Folic acid (FA) covalently linked to the coating copolymer in order to be exposed onto the nanoparticle surface was chosen as the targeting agent because folate receptors are upregulated in many cancer types. Physicochemical characterization and in vitro biocompatibility study was then performed on the prepared magnetic nanoparticles. The improved targeting and imaging properties of the prepared FA-SPIONs were further evaluated in nude mice using 7-Tesla magnetic resonance imaging (MRI). FA-SPIONs exhibited the ability to act as efficient contrast agents in conventional MRI, providing a potential nanoplatform not only for tumor diagnosis but also for cancer treatment, through the delivery of anticancer drug or locoregional magnetic hyperthermia.
Keywordsinulin copolymer superparamagnetic spinel iron oxide nanoparticles (SPIONs) magnetic resonance imaging (MRI) folic acid (FA) cancer targeting
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The authors thank Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Palermo, Italy for the use of MRI scanner. The authors also thank the MIUR and the University of Palermo for funding.
- Liu, X. L.; Ng, C. T.; Chandrasekharan, P.; Yang, H. T.; Zhao, L. Y.; Peng, E.; Lv, Y. B.; Xiao, W.; Fang, J.; Yi, J. B. et al. Synthesis of ferromagnetic Fe0.6Mn0.4O nanoflowers as a new class of magnetic theranostic platform for in vivo T 1–T 2 dual-mode magnetic resonance imaging and magnetic hyperthermia therapy. Adv. Healthc. Mater. 2016, 5, 2092–2104.CrossRefGoogle Scholar
- Wang, Y.-X. J. Superparamagnetic iron oxide based MRI contrast agents: Current status of clinical application. Quant. Imaging Med. Surg. 2011, 1, 35–40.Google Scholar
- Mauro, N.; Li Volsi, A.; Scialabba, C.; Licciardi, M.; Cavallaro, G.; Giammona, G. Photothermal ablation of cancer cells using folate-coated gold/grapheme oxide composite. Curr. Drug Deliv., in press, DOI: 10.2174/ 1567201813666160520113804.Google Scholar
- Asadov, Y. G.; Alyev, Y. I.; Jafarov, K. M. X-ray diffraction study of compounds in the Ag2S-Cu2S system. Inorg. Mater. 2008, 44, 460–466.Google Scholar
- Cavallaro, G.; Licciardi, M.; Pitarresi, G.; Giammona, G. Folate-mediated targeting of polymers as components of colloidal drug delivery systems. In Handbook of Drug Targeting and Monitoring; Andreev, B., Ed.; Nova Science Publishers Inc.: NY,2010.Google Scholar
- Lamberti, G.; Cavallaro, G.; Sardo, C.; Scialabba, C.; Licciardi, M.; Giammona, G. Smart inulin-based polycationic nanodevices for siRNA delivery. Curr. Drug Deliv. 2017, 14, 224–230.Google Scholar
- Mandracchia, D.; Denora, N.; Franco, M.; Pitarresi, G.; Giammona, G.; Trapani, G. New biodegradable hydrogels based on inulin and α,β-polyaspartylhydrazide designed for colonic drug delivery: In vitro release of glutathione and oxytocin. J. Biomater. Sci. Polym. 2011, 22, 313–328.CrossRefGoogle Scholar