Nano Research

, Volume 10, Issue 6, pp 2034–2045 | Cite as

Hot-nanoparticle-mediated fusion of selected cells

  • Azra Bahadori
  • Lene B. OddershedeEmail author
  • Poul M. BendixEmail author
Research Article


Complete fusion of two selected cells allows for the creation of novel hybrid cells with inherited genetic properties from both original cells. Alternatively, via fusion of a selected cell with a selected vesicle, chemicals or genes can be directly delivered into the cell of interest, to control cellular reactions or gene expression. Here, we demonstrate how to perform an optically controlled fusion of two selected cells or of one cell and one vesicle. Fusion is mediated by laser irradiating plasmonic gold nanoparticles optically trapped between two cells (or a vesicle and a cell) of interest. This hot-particle-mediated fusion causes total mixing of the two cytoplasms and the two cell membranes resulting in formation of a new hybrid cell with an intact cell membrane and enzymatic activity following fusion. Similarly, fusion between a vesicle and a cell results in delivery of the vesicle cargo to the cytoplasm, and after fusion, the cell shows signs of viability. The method is an implementation of targeted drug delivery at the single-cell level and has a great potential for cellular control and design.


cell fusion membrane fusion gold nanoparticle plasmonic heating optical trapping 


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The authors acknowledge financial support from the Lundbeck Foundation, the Villum Kann Rasmussen Foundation (No. VKR022593), the Danish Council for Independent Research DFF–4181-00196, from the Danish National Research Foundation (No. DNRF116) and from the Novo Nordisk Foundation (No. NNF14OC0011361).

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Hot-nanoparticle-mediated fusion of selected cells


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Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Blegdamsvej 17, Niels Bohr InstituteUniversity of CopenhagenCopenhagenDenmark
  2. 2.Lundbeck Foundation Center for Biomembranes in NanomedicineUniversity of CopenhagenCopenhagenDenmark

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