Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap
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Recently, magnetic nanoparticles (NPs) have been extensively used in food industry and biomedical treatments. However, the biocompatibility mechanism on expression proteomics, before consideration of magnetic NPs for clinical application, has not yet been fully elucidated. Therefore, this study was undertaken to identify potential biomarkers of metal ion signaling proteins in human cervical cancer cell line (HeLa) cells. Here, we report the in vitro investigations of the cell cycle response and significant changes in protein abundance of HeLa cells when exposed to self-tailored hydrophilic Fe2C NPs. The comparative proteomic approach based on 18O labeling coupled with high performance liquid chromatography/electrospray ionization with ion trap mass analyzer (HPLC/ESI-Orbitrap) was applied, and 394 proteins were identified. There were 46 significantly differentiated proteins based on the specific metal ion signaling response. Among them, 60S ribosomal protein L37a, serine/arginine-rich splicing factor 7, calmodulin, and calumenin were downregulated, whereas transketolase was overexpressed. Functional interaction network of Fe2C-regulated proteins was successfully created by the STRING algorithm to show the strong interactions between proteins. This work will not only help to understand the molecular mechanism of metal ion signaling proteins that can potentially be used to develop therapeutic protocols for diagnosis of diseases but also give direction for tailoring biocompatible magnetic NPs.
Keywordsbiocompatibility 18O labeling magnetic nanoparticles proteomic analysis
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This work was supported in part by the Research Fellowship for International Young Scientists of the National Natural Science Foundation of China (Nos. 51550110502 and 51450110437), National Natural Science Foundation of China (Nos. 81421004, 51672010, and 51590882), NSFC/RGC Joint Research Scheme (No. 51361165201), and Opening Project of Beijing National Laboratory for Molecular Science. The authors thank the mass spectrometry facility of National Center for Protein Sciences at Peking University and Dr. Wen Zhou for assistance with LC-MS analysis.
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