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Nano Research

, Volume 9, Issue 8, pp 2424–2432 | Cite as

Prolonging the plasma circulation of proteins by nano-encapsulation with phosphorylcholine-based polymer

  • Linlin Zhang
  • Yang Liu
  • Gan Liu
  • Duo Xu
  • Sheng Liang
  • Xinyuan ZhuEmail author
  • Yunfeng LuEmail author
  • Hui WangEmail author
Research Article

Abstract

Short in vivo circulation is a major hindrance to the widespread adoption of protein therapeutics. Protein nanocapsules generated by encapsulating proteins with a thin layer of phosphorylcholine-based polymer via a two-step encapsulation process exhibited significantly prolonged plasma half-life. Furthermore, by constructing nanocapsules with similar sizes but different surface charges and chemistry, we demonstrated a generic strategy for prolonging the plasma half-life of therapeutic proteins. In an in vitro experiment, four types of bovine serum albumin (BSA) nanocapsules were incubated with fetal bovine serum (FBS) in phosphate buffer saline (PBS); the cell uptake by HeLa cells was monitored to systematically evaluate the characteristics of the surface chemistry during circulation. Single positron emission tomography–computed tomography (SPECT) was employed to allow real-time observation of the BSA nanoparticle distribution in vivo, as well as quantification of the plasma concentration after intravenous administration. This study offers a practical method for translating a broad range of proteins for clinical use.

Keywords

phosphorylcholine-based polymer nano-encapsulation function protein delivery protein therapy long-circulation 

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Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of Nuclear Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong UniversityShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of Chemical and Biomolecular EngineeringUniversity of CaliforniaLos AngelesUSA
  3. 3.The Shenzhen Key Laboratory of Gene and Antibody Therapy, Center for Biotechnology and Biomedicine and Division of Life and Health SciencesTsinghua UniversityShenzhenChina
  4. 4.School of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghaiChina
  5. 5.Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer ChemistryNankai UniversityTianjinChina

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