Nano Research

, Volume 9, Issue 6, pp 1674–1681 | Cite as

Combining infrared and mode synthesizing atomic force microscopy: Application to the study of lipid vesicles inside Streptomyces bacteria

  • Pauline Vitry
  • Rolando Rebois
  • Eric Bourillot
  • Ariane Deniset-Besseau
  • Marie-Joelle Virolle
  • Eric Lesniewska
  • Alexandre Dazzi
Research Article


We propose a new analytical approach combining vibrational spectroscopy and acoustic tomography for the detection and characterization of vesicles inside Streptomyces bacteria. Using atomic force microscopy and infrared spectroscopy (AFM-IR), we detect the presence of triglyceride vesicles. Their sizes in depth are measured with high accuracy using mode synthesizing atomic force microscopy (MS-AFM). We conducted a comparative study of AFM-IR and MS-AFM, and highlighted the advantages of the coupling of these techniques in having a full characterization (chemical, topographical, and volumetric) of a biological sample. With these complementary techniques, a complete access to the vesicle size distribution has been achieved with an accuracy of less than 50 nm. A 3D reconstruction of bacteria showing the in-depth distribution of vesicles is given to underline the great potential of the acoustic method.


nanoscale subsurface imaging atomic force microscopy acoustic microscopy infrared microscopy biology 


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Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Pauline Vitry
    • 1
  • Rolando Rebois
    • 2
  • Eric Bourillot
    • 1
  • Ariane Deniset-Besseau
    • 2
  • Marie-Joelle Virolle
    • 3
  • Eric Lesniewska
    • 1
  • Alexandre Dazzi
    • 2
  1. 1.ICB UMR CNRS 6303University of Bourgogne Franche ComtéDijonFrance
  2. 2.LCP UMR CNRS 8000University Paris-SudOrsayFrance
  3. 3.I2BC UMR CNRS 9198University Paris-SudGif-Sur-YvetteFrance

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