Nano Research

, Volume 7, Issue 11, pp 1659–1669 | Cite as

Enhanced photodynamic therapy of mixed phase TiO2(B)/anatase nanofibers for killing of HeLa cells

  • Shuchao Zhang
  • Dongjiang Yang
  • Dengwei Jing
  • Hongwei Liu
  • Long Liu
  • Yi Jia
  • Meihua Gao
  • Liejin Guo
  • Ziyang Huo
Research Article


Photodynamic therapy (PDT), which is a procedure that uses photosensitizing drug to apply therapy selectively to target sites, has been proven to be a safe treatment for cancers and conditions that may develop into cancers. Nano-sized TiO2 has been regarded as potential photosensitizer for UV light driven PDT. In this study, four types of TiO2 nanofibers were prepared from proton tri-titanate (H2T3O7) nanofiber. The as-obtained nanofibers were demonstrated as efficient photosensitizers for PDT killing of HeLa cells. MTT assay and flow cytometry (FCM) were carried out to evaluate the biocompatibility, percentage of apoptotic cells, and cell viability. The non-cytotoxicity of the as-prepared TiO2 nanofibers in the absence of UV irradiation has also been demonstrated. Under UV light irradiation, the TiO2 nanofibers, particularly the mixed phase nanofibers, displayed much higher cell-killing efficiency than Pirarubicin (THP), which is a common drug to induce the apoptosis of HeLa cells. We ascribe the high cellkilling efficiency of the mixed phase nanofibers to the bandgap edge match and stable interface between TiO2(B) and anatase phases in a single nanofiber, which can inhibit the recombination of the photogenerated electrons and holes. This promotes the charge separation and transfer processes and can produce more reactive oxygen species (ROS) that are responsible for the killing of HeLa cells.


photodynamic therapy titanium oxides (TiO2nanomaterials apoptosis mixed phase 


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Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Blood Transfusion, the Affiliated Hospital of Medical CollegeQingdao UniversityQingdaoChina
  2. 2.Department of ImmunologyMedical College of Qingdao UniversityQingdaoChina
  3. 3.Collaborative Innovation Centre for Marine Biomass Fibers, Materials and Textiles of Shandong Province; College of Chemical and Environmental EngineeringQingdao UniversityQingdaoChina
  4. 4.Queensland Micro- and Nanotechnology Centre (QMNC)Griffith University, NathanBrisbaneAustralia
  5. 5.International Research Center for Renewable Energy, State Key Laboratory of Multiphase Flow in Power Engineering, College of Chemistry, Chemical and Environmental EngineeringXi’an Jiaotong UniversityXi’anChina
  6. 6.Australian Centre for Microscopy and Microanalysis (ACMM)the University of SydneySydneyAustralia

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