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High-throughput genetic screens using CRISPR–Cas9 system

Archives of Pharmacal Research volume 41, pages 875–884 (2018)Cite this article

Abstract

The CRISPR–Cas9 system is a powerful tool for genome engineering, and its programmability and simplicity have enabled various types of gene manipulation such as gene disruption and transcriptional and epigenetic perturbation. Particularly, CRISPR-based pooled libraries facilitate high-throughput screening for functional regulatory elements in the human genome. In this review, we describe recent advances in CRISPR–Cas9 technology and its use in high-throughput genetic screening. We also discuss its potential for drug target discovery and current challenges of this technique in biomedical research.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (Y.K., NRF-2016R1D1A1A02937096, J.K., NRF-2016R1A6A3A04009014) and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Y.K., HI17C0337).

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  1. Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea

    Jiyeon Kweon & Yongsub Kim

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  1. Jiyeon Kweon
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  2. Yongsub Kim
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Correspondence to Yongsub Kim.

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Kweon, J., Kim, Y. High-throughput genetic screens using CRISPR–Cas9 system. Arch. Pharm. Res. 41, 875–884 (2018). https://doi.org/10.1007/s12272-018-1029-z

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Keywords

  • CRISPR–Cas9 system
  • Genome engineering
  • High-throughput screening
  • Pooled CRISPR screens