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Comparison of the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide on Japanese encephalitis vaccine in mice

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Abstract

Adjuvants are essential vaccine components used to enhance, accelerate, and/or prolong adaptive immunity against specific vaccine antigens. In this study, we compared the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide (dLOS), a toll-like receptor 4 agonist, on the Japanese encephalitis (JE) vaccine in mice. Mice were immunized once or twice at a two-week interval with inactivated JE vaccine in the absence or presence of adjuvant. We found that both the alum- and the liposome-based formulation induced significantly faster and higher serum IgG antibody responses as compared with the non-adjuvanted vaccine after either one or two immunizations. The antibody titers of the mouse immune sera correlated with 50% plaque reduction neutralization test (PRNT50) antibody titers. In addition, the dLOS/liposome formulation was more effective in inducing a Th1-type immune response than the dLOS/alum formulation, as suggested by a strong antigen-specific interferon (IFN)-γ response. Based on these results, we suggest that both alum- and liposome-based adjuvant formulations containing dLOS may be used for the development of JE vaccines with improved immunogenicity.

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Acknowledgements

We thank Prof. J.H. Nam of Catholic University and Prof. B.L. Seong of Yonsei University (Republic of Korea) for providing the JEV Nakayama strain and recombinant JEV E protein, respectively. This study was supported by grants from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (No. HI14C2664 and No. HI13C0826).

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  1. Ara Ko and Seo Ri Wui have contributed equally to this work.

Authors and Affiliations

  1. Department of Bioscience & Biotechnology, Sejong University, 98 Kunja-dong, Kwangjin-gu, Seoul, 05006, Republic of Korea

    Ara Ko, Seo Ri Wui, Ji In Ryu, Hien Thi Thu Do, Yeon Jeong Lee, Soo Jeong Lim, Inmoo Rhee & Na Gyong Lee

  2. Laboratory Science Division, International Vaccine Institute, Seoul, 08826, Republic of Korea

    Dae Im Jung, Jin-ah Park, Jung-ah Choi & Man Ki Song

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  1. Ara Ko
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  2. Seo Ri Wui
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Correspondence to Inmoo Rhee or Na Gyong Lee.

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Conflict of interest

NG. Lee is an inventor of EyeGene-owned patents on dLOS-based adjuvants and a scientific advisor for EyeGene. The other authors have no conflicts of interests.

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Ko, A., Wui, S.R., Ryu, J.I. et al. Comparison of the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide on Japanese encephalitis vaccine in mice. Arch. Pharm. Res. 41, 219–228 (2018). https://doi.org/10.1007/s12272-017-0985-z

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