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Effect of processing method on platycodin D content in Platycodon grandiflorum roots

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Abstract

Platycodon grandiflorum root is a traditional medicine and food material rich in triterpenoid saponins. Its major constituent, platycodin D (PD), is known to have various pharmacological properties, but processing methods may influence the PD content. In this study, a fully validated HPLC–ELSD method was developed for the quantification of PD in various states of 73 P. grandiflorum root samples from East Asia, and it exhibited a marked variation of the content. Furthermore, the effects of processing procedures such as peeling and drying temperature on the PD content were investigated using UPLC–ELSD analysis, and as a result, a significant influence of processing methods such as peeling and heating of samples on the content was confirmed. Specifically, unpeeled samples that were dried at 40 °C showed the greatest PD content. The obtained results could facilitate the reliable standardization of P. grandiflorum for precise authentication and efficacious applications.

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Acknowledgements

This work was supported by a grant from the National Research Foundation of Korea (NRF-2015R1D1A1A01060321).

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Authors and Affiliations

  1. Natural Constituents Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, 25451, Korea

    Jaeyoung Kwon

  2. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea

    Hyaemin Lee & Dongho Lee

  3. Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju, 36040, Korea

    Nahyun Kim

  4. College of Oriental Medicine, Dongguk University, Gyeongju, 38066, Korea

    Je-Hyun Lee

  5. College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Korea

    Mi Hee Woo

  6. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 08826, Korea

    Jinwoong Kim

  7. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 08826, Korea

    Yeong Shik Kim

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  1. Jaeyoung Kwon
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  2. Hyaemin Lee
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Correspondence to Dongho Lee.

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Kwon, J., Lee, H., Kim, N. et al. Effect of processing method on platycodin D content in Platycodon grandiflorum roots. Arch. Pharm. Res. 40, 1087–1093 (2017). https://doi.org/10.1007/s12272-017-0946-6

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  • DOI: https://doi.org/10.1007/s12272-017-0946-6

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