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Neuroprotective compounds from Reynoutria sachalinensis

Archives of Pharmacal Research volume 40, pages 704–712 (2017)Cite this article

Abstract

Glutamate is a neurotransmitter in central nervous system. Overexpression of glutamate leads to oxidative stress, resulting in several neurodegenerative disorders that include Alzheimer’s disease. The n-hexane fraction of stems and ethyl acetate (EtOAc) fraction of flowers of Reynoutria sachalinensis provide neuroprotection against glutamate-induced oxidative toxicity in HT22 cells. In this study, 1-decanol (1), β-amyrin (2), dammaran-3β-ol (3), campesterol (4), daucosterol (5), ergosterol peroxide (6), emodin 8-O-β-d-glucopyranoside (7), quercetin (8) and isoquercitrin (9) were isolated from n-hexane fractions of stems and EtOAc fractions of flowers of R. sachalinensis. Their neuroprotective activity was evaluated by MTT assay. 1-Decanol, campesterol, ergosterol peroxide, quercetin and isoquercitrin exhibited neuroprotective activity. These compounds decreased reactive oxygen species level, showed anti-oxidant activity with DPPH radical and in a H2O2 scavenging assay. Therefore, the neuroprotective activity of 1-decanol, campesterol, ergosterol peroxide, quercetin and isoquercitrin are associated with antioxidant activity.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science, ICT & Future Planning) (No. 2016R1A2B1011384).

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Affiliations

  1. Department of Medical Biomaterials Engineering, College of Biomedical science, Kangwon National University, Hyoja-2 Dong, Chuncheon, 200-701, Republic of Korea

    Min Rye Eom, Jin Bae Weon, Youn Sik Jung, Ga Hee Ryu, Woo Seung Yang & Choong Je Ma

  2. Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Choong Je Ma

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  1. Min Rye Eom
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  2. Jin Bae Weon
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  3. Youn Sik Jung
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  4. Ga Hee Ryu
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Correspondence to Choong Je Ma.

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Eom, M.R., Weon, J.B., Jung, Y.S. et al. Neuroprotective compounds from Reynoutria sachalinensis . Arch. Pharm. Res. 40, 704–712 (2017). https://doi.org/10.1007/s12272-017-0918-x

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  • DOI: https://doi.org/10.1007/s12272-017-0918-x

Keywords

  • R. sachalinensis
  • Glutamate
  • Neuroprotective activity
  • Anti-oxidant activity