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Archives of Pharmacal Research

, Volume 39, Issue 12, pp 1739–1747 | Cite as

The cytoprotective effect of Rumex Aquaticus Herba extract against hydrogen peroxide-induced oxidative stress in AGS cells

  • Eun Jeong Cho
  • Seung In Um
  • Jeong Hoon Han
  • Byeonghee Kim
  • Sang Beom Han
  • Ji Hoon Jeong
  • Hak Rim Kim
  • Inkyeom Kim
  • Wan Kyun Whang
  • Eunhwa Lee
  • Uy Dong SohnEmail author
Research Article

Abstract

The Rumex Aquaticus Herba extract containing quercetin-3-β-D-glucuronopyranoside (ECQ) has been reported to exhibit various pharmacological activities, including anti-inflammatory and anti-oxidative effects. This plant has been traditionally used for the treatment of diarrhea, disinfestation, edema and jaundice, and as an antipyretic drug. The aim of the present study was to investigate the ability of ECQ to protect against oxidative damage and to determine its signaling mechanism in AGS cells. The protein expressions of heme oxygenase-1 (HO-1) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were measured by Western blots. Cell viability was measured by MTT assay. Intracellular reactive oxygen species (ROS) levels were measured using 2′,7′-dichlorofluorescein diacetate. Glutathione peroxidase levels were measured using kits. The protein expressions of HO-1 and its upstream mediator, Nrf2, increased after ECQ treatment. The HO-1 inhibitor, ZnPP, repressed the protective effect of ECQ on H2O2-induced cell damage. We found that LY294002, a specific PI3 K/Akt inhibitor, suppressed ECQ-induced HO-1 expression. ECQ significantly attenuated H2O2-induced cytotoxicity and ROS generation. Also, ECQ enhanced the antioxidant enzyme activities of glutathione peroxidase. These results suggest that ECQ exerts a cytoprotective effect against H2O2-induced oxidative stress by upregulation of Nrf2/HO-1 via the PI3 K/Akt pathway.

Keywords

Herbal extract Oxidative stress Antioxidant Anti-inflammatory Cytoprotection 

Notes

Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology [Grant 2016R1D1A1A09918019].

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.

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Copyright information

© The Pharmaceutical Society of Korea 2016

Authors and Affiliations

  • Eun Jeong Cho
    • 1
  • Seung In Um
    • 1
  • Jeong Hoon Han
    • 1
  • Byeonghee Kim
    • 1
  • Sang Beom Han
    • 1
  • Ji Hoon Jeong
    • 2
  • Hak Rim Kim
    • 3
  • Inkyeom Kim
    • 4
  • Wan Kyun Whang
    • 1
  • Eunhwa Lee
    • 5
  • Uy Dong Sohn
    • 1
    Email author
  1. 1.College of PharmacyChung-Ang UniversitySeoulRepublic of Korea
  2. 2.College of MedicineChung-Ang UniversitySeoulRepublic of Korea
  3. 3.College of MedicineDankook UniversityCheonanRepublic of Korea
  4. 4.College of MedicineKyungpook National UniversityDaeguRepublic of Korea
  5. 5.The Graduate School of Pharmaceutical Industry ManagementChung-Ang UniversitySeoulRepublic of Korea

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